Photobiomodulation (blue and green light) encourages osteoblastic-differentiation of human adipose-derived stem cells: role of intracellular calcium and light-gated ion channels
Wang Y, Huang YY, Wang Y, Lyu P, Hamblin MR
Scientific reports · 150 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Institutes of Health
- Government
- National Institute of Allergy and Infectious Diseases
- Government
- NIAID NIH HHS
- Grants
- National Institute of Allergy and Infectious Diseases (R01 AI050875); National Institutes of Health (AI050875)
Based on 3 listed funder(s).
Publication
- Published
- 2016-09-21 · Sci Rep · vol. 6 · issue 1 · p. 33719
- Publisher
- Nature Portfolio
- Cited
- 226 citations · more than 99% of similar papers · 13.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 54 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Laser Applications in Dentistry and Medicine · Planarian Biology and Electrostimulation · Photoreceptor and optogenetics research
- Keywords
- Osteocalcin, Osteoblast, Calcium, Chemistry, Stem cell, Cell biology, Intracellular, Biophysics, Adipose tissue, Cellular differentiation, Alkaline phosphatase, In vitro, Biology, Biochemistry
- MeSH
- adipose tissue, osteoblasts, stem cells, humans, calcium, ion channels, antigens, differentiation, lasers, cell differentiation, ion channel gating, light
5 authors
From US, CN
- Yuguang WangHarvard University; Peking University; Massachusetts General Hospital
- Ying‐Ying HuangHarvard University; Massachusetts General Hospital
- Yong WangHarvard University; Peking University; Massachusetts General Hospital
- Peijun LyuPeking University
- Michael R. HamblinHarvard University; Massachusetts General Hospital; Harvard–MIT Division of Health Sciences and Technology
Abstract
Unlabelled
Human adipose-derived stem cells (hASCs) have the potential to differentiate into several different cell types including osteoblasts. Photobiomodulation (PBM) or low level laser therapy (LLLT) using red or near-infrared wavelengths has been reported to have effects on both proliferation and osteogenic differentiation of stem cells. We examined the effects of delivering four different wavelengths (420 nm, 540 nm, 660 nm, 810 nm) at the same dose (3 J/cm(2)) five times (every two days) on hASCs cultured in osteogenic medium over three weeks. We measured expression of the following transcription factors by
Rt-pcr
RUNX2, osterix, and the osteoblast protein, osteocalcin. The 420 nm and 540 nm wavelengths were more effective in stimulating osteoblast differentiation compared to 660 nm and 810 nm. Intracellular calcium was higher after 420 nm and 540 nm, and could be inhibited by capsazepine and SKF96365, which also inhibited osteogenic differentiation. We hypothesize that activation of light-gated calcium ion channels by blue and green light could explain our results.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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