Physiologic changes of urinary proteome by caffeine and excessive water intake
Peerapen P, Ausakunpipat N, Sutthimethakorn S, Aluksanasuwan S, Vinaiphat A, Thongboonkerd V
Clinical chemistry and laboratory medicine · 11 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Controlled clinical trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2016-12-17 · Clin Chem Lab Med · vol. 55 · issue 7 · pp. 993–1002
- Publisher
- De Gruyter
- Cited
- 17 citations · more than 85% of similar papers · 1.7× the field average
- References
- 44 works
- Access
- Paywalled
- Research areas
- Coffee research and impacts · Circadian rhythm and melatonin · Urinary Bladder and Prostate Research
- Keywords
- Urinary system, Proteome, Urine, Blot, Chemistry, Proteomics, Internal medicine, Endocrinology, Biochemistry, Medicine
- MeSH
- humans, water, caffeine, proteome, urinalysis, artifacts, drinking, dose-response relationship, drug, time factors, adult, female, male
6 authors
From TH
- Paleerath PeerapenSiriraj Hospital; Mahidol University
- Nardtaya AusakunpipatSiriraj Hospital; Mahidol University
- Suchitra SutthimethakornSiriraj Hospital; Mahidol University
- Siripat AluksanasuwanSiriraj Hospital; Mahidol University
- Arada VinaiphatSiriraj Hospital; Mahidol University
- Visith Thongboonkerd · correspondingSiriraj Hospital; Mahidol University
Abstract
Background
Diurnal variations and physiologic changes of urinary proteome have been suggested in the urinary proteomics field. However, no clear evidence has been demonstrated. The present study thus aimed to define changes in urinary proteome by physiological stimuli, i.e. caffeine intake and excessive water drinking, both of which cause physiologic diuresis.
Methods
Urine samples were collected from 30 healthy individuals under three different conditions: (i) morning void as the control; (ii) after drinking a cup of coffee; and (iii) after drinking 1 L of water within 20 min. Thereafter, differentially excreted proteins were analyzed by 2-DE proteomics approach and validated by Western blotting and ELISA.
Results
Spot matching, quantitative intensity analysis, and ANOVA followed by Tukey's post-hoc multiple comparisons and the Bonferroni correction revealed significant differences in levels of five protein spots among three different conditions. These proteins were identified by quadrupole time-of-flight mass spectrometry (Q-TOF MS) and/or MS/MS analyses as kininogen 1 isoform 3, β-actin, prostaglandin D synthase (PGDS), fibrinogen α-chain and immunoglobulin light chain. Among these, the decreased level of immunoglobulin was successfully validated by Western blotting and ELISA.
Conclusions
These data indicated that caffeine intake and excessive water drinking could affect urinary excretion of some proteins and may affect urinary proteome analysis.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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