Low-Level Laser Effect on Proliferation, Migration, and Antiapoptosis of Mesenchymal Stem Cells
Yin K, Zhu R, Wang S, Zhao RC
Stem cells and development · 74 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Natural Science Foundation of China
Based on 1 listed funder(s).
Publication
- Published
- 2017-02-09 · Stem Cells Dev · vol. 26 · issue 10 · pp. 762–775
- Publisher
- Mary Ann Liebert, Inc.
- Cited
- 92 citations · more than 97% of similar papers · 6.5× the field average
- Impact
- Top 10% most cited in its field
- References
- 41 works
- Access
- Paywalled
- Research areas
- Laser Applications in Dentistry and Medicine · Laser-Ablation Synthesis of Nanoparticles · Laser Material Processing Techniques
- Keywords
- Biology, Mesenchymal stem cell, Cell biology, Viability assay, Cell, Immunocytochemistry, Molecular biology, Biochemistry
- MeSH
- adipose tissue, cells, cultured, cytoskeleton, mitochondria, mesenchymal stem cells, humans, lasers, apoptosis, cell proliferation, cell movement, light, low-level light therapy
4 authors
From CN
- Kan YinChinese Academy of Medical Sciences & Peking Union Medical College; Peking Union Medical College Hospital
- Rongjia ZhuChinese Academy of Medical Sciences & Peking Union Medical College; Peking Union Medical College Hospital
- Shihua WangChinese Academy of Medical Sciences & Peking Union Medical College; Peking Union Medical College Hospital
- Robert Chunhua ZhaoChinese Academy of Medical Sciences & Peking Union Medical College; Peking Union Medical College Hospital
Abstract
Mesenchymal stem cells (MSCs) have been proved to be an important element in cell-based therapy. Photobiomodulation used extremely low-level lasers (LLLs) to affect the behavior of cells. The effect mechanism of LLLs on MSCs from human remained to be discovered. In this study, cell viability was assessed using MTS assays and cell cycle was evaluated by fluorescence-activated cell sorting (FACS). The influence of LLLs on mitochondrial biogenesis (fission or fusion) and function (ATP, reactive oxygen species [ROS], nitric oxide [NO]) was evaluated by transmission electron microscope, FACS, quantitative real time polymerase chain reaction (q-PCR), and immunocytochemistry. Cell migration and cytoskeleton alteration (actin and tubulin) were evaluated using transwell assay, immunocytochemistry, enzyme-linked immunosorbent assay, and western blotting. Cell apoptosis was evaluated using FACS, immunocytochemistry, and western blotting. We investigated that certain influence of LLLs on MSCs in vitro 6 or 24 h after 1 h of LLL irradiation. The mechanism of the effects included proliferation rate increase mediated by increased S phase proportion; mitochondrial biogenesis and function alteration mediated by fusion (Mfn1, Mfn2, and Opa-1) and fission (Fis1, Drp-1, and MTP18)-related proteins, NRF1, TFAM, PGC-1a, and upregulated intracellular ROS and NO concentration; migration acceleration through the ERK1/2 and FAK pathway and upregulation of growth factors such as HGF and PDGF; and resistance to apoptosis with increased Bcl-2 and decreased Bax, or through tunneling nanotube formation between LLL-treated MSCs and 5-fluorouracil-induced apoptotic MSCs. These observations suggested that LLLs enhanced stem cell survival and therapeutic function, which could appear to be an innovative pretreatment in the application of MSCs.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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