Broccoli-Derived Nanoparticle Inhibits Mouse Colitis by Activating Dendritic Cell AMP-Activated Protein Kinase
Deng Z, Rong Y, Teng Y, Mu J, Zhuang X, Tseng M, Samykutty A, Zhang L, Yan J, Miller D, Suttles J, Zhang HG
Molecular therapy : the journal of the American Society of Gene Therapy · 388 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Clinical events such as disease or death
Who paid for it
- Funding
- Independent funding
- Government
- U.S. Department of Veterans Affairs
- Government
- China Scholarship Council
- Government
- National Institutes of Health
- Government
- National Institute of General Medical Sciences
- Government
- National Center for Advancing Translational Sciences
- Government
- National Center for Complementary and Integrative Health
- Government
- BLRD VA
- Government
- NIGMS NIH HHS
- Government
- NCCIH NIH HHS
- Government
- Robley Rex VA Medical Center
- Government
- NCATS NIH HHS
- Government
- NIH
- Government
- Research Career Scientist (RCS) Award
- Unclassified
- Institutional Development Award
- Grants
- China Scholarship Council (201406275038); National Center for Complementary and Integrative Health (R01 AT008617); National Institute of General Medical Sciences (P20 GM113226); U.S. Department of Veterans Affairs (I01 BX000962); National Center for Advancing Translational Sciences (UH3TR000875); National Institute of General Medical Sciences (P20GM113226-6174); National Institutes of Health (UH3TR000875); National Institutes of Health (R01AT008617)
Based on 14 listed funder(s).
Publication
- Published
- 2017-03-07 · Mol Ther · vol. 25 · issue 7 · pp. 1641–1654
- Publisher
- Elsevier BV
- Cited
- 502 citations · more than 96% of similar papers · 5.0× the field average
- Impact
- Top 10% most cited in its field
- References
- 49 works
- Access
- Open access (hybrid journal) · CC-BY-NC-ND
- Research areas
- Immunotherapy and Immune Responses · Immune Cell Function and Interaction · Psoriasis: Treatment and Pathogenesis
- Keywords
- AMPK, Immune system, Colitis, Protein kinase A, Cell biology, Adoptive cell transfer, AMP-activated protein kinase, Cancer research, Biology, Chemistry, Immunology, Kinase, T cell
- MeSH
- dendritic cells, animals, mice, inbred c57bl, humans, mice, brassica, colitis, ulcerative, disease models, animal, sodium dodecyl sulfate, isothiocyanates, sulfoxides, lipids, anti-inflammatory agents, plant extracts, adoptive transfer, administration, oral, immune tolerance, gene expression, enzyme activation, nanoparticles, amp-activated protein kinases
12 authors
From US
- Zhongbin DengUniversity of Louisville
- Yuan RongUniversity of Louisville
- Yun TengUniversity of Louisville
- Jingyao MuUniversity of Louisville
- Xiaoying ZhuangUniversity of Louisville
- Michael T. TsengUniversity of Louisville
Abstract
The intestinal immune system is continuously exposed to massive amounts of nanoparticles derived from food. Whether nanoparticles from plants we eat daily have a role in maintaining intestinal immune homeostasis is poorly defined. Here, we present evidence supporting our hypothesis that edible nanoparticles regulate intestinal immune homeostasis by targeting dendritic cells (DCs). Using three mouse colitis models, our data show that orally given nanoparticles isolated from broccoli extracts protect mice against colitis. Broccoli-derived nanoparticle (BDN)-mediated activation of adenosine monophosphate-activated protein kinase (AMPK) in DCs plays a role in not only prevention of DC activation but also induction of tolerant DCs. Adoptively transferring DCs pre-pulsed with total BDN lipids, but not sulforaphane (SFN)-depleted BDN lipids, prevented DSS-induced colitis in C57BL/6 (B6) mice, supporting the role of BDN SFN in the induction of DC tolerance. Adoptively transferring AMPK+/+, but not AMPK-/-, DCs pre-pulsed with SFN prevented DSS-induced colitis in B6 mice, further supporting the DC AMPK role in SFN-mediated prevention of DSS-induced colitis. This finding could open new preventive or therapeutic avenues to address intestinal-related inflammatory diseases via activating AMPK.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY-NC-ND).
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