A Fermented Whole Grain Prevents Lipopolysaccharides-Induced Dysfunction in Human Endothelial Progenitor Cells
Giusti L, Gabriele M, Penno G, Garofolo M, Longo V, Del Prato S, Lucchesi D, Pucci L
Oxidative medicine and cellular longevity · 28 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Regione Toscana
- Grants
- Regione Toscana (D55E11002680005)
Based on 1 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2017-01-01 · Oxid Med Cell Longev · vol. 2017 · issue 1 · p. 1026268
- Publisher
- Hindawi Publishing Corporation
- Cited
- 40 citations · more than 81% of similar papers · 1.6× the field average
- References
- 49 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Immune cells in cancer · Adipokines, Inflammation, and Metabolic Diseases · Inflammation biomarkers and pathways
- Keywords
- Proinflammatory cytokine, Progenitor cell, Cell biology, Unfolded protein response, Oxidative stress, Viability assay, Endothelial dysfunction, Inflammation, Chemistry, NF-κB, Signal transduction, Apoptosis, Stem cell, Endoplasmic reticulum, Biology, Immunology, Endocrinology, Biochemistry
- MeSH
- leukocytes, mononuclear, humans, reactive oxygen species, lipopolysaccharides, plant preparations, cell survival, fermentation, real-time polymerase chain reaction, endothelial progenitor cells, whole grains
8 authors
From IT
- Laura GiustiUniversity of Pisa; Azienda Ospedaliera Universitaria Pisana
- Morena GabrieleNational Research Council; Deleted Institution
- Giuseppe PennoUniversity of Pisa; Azienda Ospedaliera Universitaria Pisana
- Monia GarofoloUniversity of Pisa; Azienda Ospedaliera Universitaria Pisana
- Vincenzo G. Longo · correspondingNational Research Council; Deleted Institution
- Stefano Del PratoUniversity of Pisa; Azienda Ospedaliera Universitaria Pisana
Abstract
Endogenous and exogenous signals derived by the gut microbiota such as lipopolysaccharides (LPS) orchestrate inflammatory responses contributing to development of the endothelial dysfunction associated with atherosclerosis in obesity, metabolic syndrome, and diabetes. Endothelial progenitor cells (EPCs), bone marrow derived stem cells, promote recovery of damaged endothelium playing a pivotal role in cardiovascular repair. Since healthy nutrition improves EPCs functions, we evaluated the effect of a fermented grain, Lisosan G (LG), on early EPCs exposed to LPS. The potential protective effect of LG against LPS-induced alterations was evaluated as cell viability, adhesiveness, ROS production, gene expression, and NF-kB signaling pathway activation. Our results showed that LPS treatment did not affect EPCs viability and adhesiveness but induced endothelial alterations via activation of NF-kB signaling. LG protects EPCs from inflammation as well as from LPS-induced oxidative and endoplasmic reticulum (ER) stress reducing ROS levels, downregulating proinflammatory and proapoptotic factors, and strengthening antioxidant defense. Moreover, LG pretreatment prevented NF-kB translocation from the cytoplasm into the nucleus caused by LPS exposure. In human EPCs, LPS increases ROS and upregulates proinflammatory tone, proapoptotic factors, and antioxidants. LG protects EPCs exposed to LPS reducing ROS, downregulating proinflammatory and proapoptotic factors, and strengthening antioxidant defenses possibly by inhibiting NF-κB nuclear translocation.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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