A Direct Assay for Measuring Free 25-Hydroxyvitamin D
Heureux N, Lindhout E, Swinkels L
Journal of AOAC International · 37 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2017-05-11 · J AOAC Int · vol. 100 · issue 5 · pp. 1318–1322
- Publisher
- Oxford University Press
- Cited
- 49 citations · more than 96% of similar papers · 4.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 0 works
- Access
- Paywalled
- Research areas
- Vitamin D Research Studies · HIV-related health complications and treatments · Pharmacological Effects and Toxicity Studies
- Keywords
- Vitamin D-binding protein, Immunoassay, Vitamin D and neurology, Albumin, Hemodialysis, Medicine, Dialysis, Internal medicine, Vitamin, Chemistry, Chemiluminescent immunoassay, Chromatography, Endocrinology, Gastroenterology, Antibody, Immunology
- MeSH
- animals, humans, mice, vitamin d, blood chemical analysis, enzyme-linked immunosorbent assay, limit of detection
3 authors
From NL
- Nicolas Heureux · corresponding
- Ernst LindhoutFuture Diagnostics (Netherlands)
- L M SwinkelsFuture Diagnostics (Netherlands)
Abstract
Recent studies suggest that the concentration and genotype of vitamin D binding protein (VDBP) are important factors that determine the bioavailability of 25-hydroxyvitamin D [25(OH)D] in blood. Accumulating data indicate that, e.g., in pregnant women, hemodialysis patients, chronic kidney disease, liver failure, and bladder and pancreatic cancers, the measurement of free 25(OH)D in serum provides more relevant diagnostic information than measurement of total 25(OH)D. The aim of this study was to develop and validate an ELISA for direct measurement of free 25(OH)D in serum. A simple and direct ELISA was developed, based on a two-step immunoassay procedure performed in a microtiter plate. The assay has been characterized in terms of precision (4-10% CV, according to concentration), sensitivity (limits of blank = 0.5-1.0 pg/mL and LODs = 1.3-1.8 pg/mL), accuracy (correlation to dialysis, ELISA = 0.99xdialysis-0.5 pg/mL, r2 = 0.74), cross-reactivity of the antibody for the D2 form (77%), and addition of both VDBP and albumin (35-38% recovery upon addition of VDBP, 53-58% upon addition of albumin). The assay has already been used in multiple studies, including its comparison with calculation methods and in studies of patients with liver failure, different ethnic groups, supplemented mice, respiratory diseases, and obesity. The free 25(OH)D ELISA can be used in studies as a valuable tool to establish the clinical relevance of free 25(OH)D.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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