Caffeine induces sustained apoptosis of human gastric cancer cells by activating the caspase‑9/caspase‑3 signalling pathway
Liu H, Zhou Y, Tang L
Molecular medicine reports · 49 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Government of Jiangsu Province
Based on 1 listed funder(s).
Publication
- Published
- 2017-03-01 · Mol Med Rep · vol. 16 · issue 3 · pp. 2445–2454
- Publisher
- Spandidos Publishing
- Cited
- 77 citations · more than 95% of similar papers · 4.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 71 works
- Access
- Open access (hybrid journal) · CC-BY-NC-ND
- Research areas
- Coffee research and impacts · Tea Polyphenols and Effects · Adenosine and Purinergic Signaling
- Keywords
- Caffeine, Apoptosis, Cell cycle, Viability assay, Cancer cell, Cancer, Caspase, Cell growth, Pharmacology, Caspase 3, Cell, Cancer research, Flow cytometry, Oncogene, Chemistry, Biology, Programmed cell death, Immunology, Biochemistry, Endocrinology
- MeSH
- cell line, tumor, humans, stomach neoplasms, caffeine, antineoplastic agents, signal transduction, cell cycle, apoptosis, enzyme activation, caspase 9, caspase 3
3 authors
From CN
- Hanyang LiuChangzhou No.2 People's Hospital; Nanjing Medical University
- Yan ZhouChangzhou No.2 People's Hospital; Nanjing Medical University
- Liming TangChangzhou No.2 People's Hospital; Nanjing Medical University
Abstract
Caffeine is one of the most widely consumed substances found in beverages, and has demonstrated anticancer effects in several types of cancer. The present study aimed to examine the anticancer effects of caffeine on gastric cancer (GC) cells (MGC‑803 and SGC‑7901) in vitro, and to determine whether the apoptosis‑related caspase‑9/-3 pathway is associated with these effects. The sustained antiproliferative effects of caffeine on gastric cancer were also investigated. GC cell viability and proliferation were evaluated using cell counting and colony forming assays, following treatment with various concentrations of caffeine. Flow cytometry was performed to assess cell cycle dynamics and apoptosis. Western blot analysis was conducted to detect the activity of the caspase‑9/-3 pathway. The results indicated that caffeine treatment significantly suppressed GC cell growth and viability and induced apoptosis by activating the caspase‑9/-3 pathway. Furthermore, the anticancer effects of caffeine appeared to be sustained, as the caspase‑9/-3 pathway remained active following caffeine withdrawal. In conclusion, caffeine may function as a sustained anticancer agent by activating the caspase‑9/-3 pathway, which indicates that it may be useful as a therapeutic candidate in gastric cancer.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY-NC-ND).
Community trust
Loading…
How much do you trust this study's findings?
Comments
Sign in to rate, comment on or flag this study.Sign inSomething wrong here?
Flag this study if its information, labels or funding look wrong. An editor reviews every flag.
Sign in to rate, comment on or flag this study.Sign inEducational information about published research. Not medical advice, and not a recommendation to start or stop anything.