Red (660 nm) or near-infrared (810 nm) photobiomodulation stimulates, while blue (415 nm), green (540 nm) light inhibits proliferation in human adipose-derived stem cells
Wang Y, Huang YY, Wang Y, Lyu P, Hamblin MR
Scientific reports · 136 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Natural Science Foundation of China
- Government
- National Institutes of Health
- Government
- National Institute of Allergy and Infectious Diseases
- Government
- NIAID NIH HHS
- Grants
- National Institute of Allergy and Infectious Diseases (R01 AI050875); National Institute of Allergy and Infectious Diseases (R21 AI121700); National Institutes of Health (R01AI050875); National Institutes of Health (R21AI121700)
Based on 4 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2017-08-04 · Sci Rep · vol. 7 · issue 1 · p. 7781
- Publisher
- Nature Portfolio
- Cited
- 209 citations · more than 99% of similar papers · 11.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 46 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Laser Applications in Dentistry and Medicine · Hydrogen's biological and therapeutic effects · Spaceflight effects on biology
- Keywords
- Capsazepine, Chemistry, Reactive oxygen species, Green-light, Intracellular, Calcium in biology, Biophysics, Calcium, Cell biology, TRPV1, Transient receptor potential channel, Biochemistry, Receptor, Biology, Red light, Botany
- MeSH
- adipose tissue, cells, cultured, mesenchymal stem cells, humans, calcium, reactive oxygen species, capsaicin, adenosine triphosphate, infrared rays, ultraviolet rays, cell proliferation, trpv cation channels, membrane potential, mitochondrial
5 authors
From US, CN
- Yuguang WangHarvard University; Peking University; Massachusetts General Hospital
- Ying‐Ying HuangHarvard University; Massachusetts General Hospital
- Yong WangHarvard University; Peking University; Massachusetts General Hospital
- Peijun Lyu · correspondingKing University
- Michael R. Hamblin · correspondingHarvard University; Massachusetts General Hospital; Harvard–MIT Division of Health Sciences and Technology
Abstract
We previously showed that blue (415 nm) and green (540 nm) wavelengths were more effective in stimulating osteoblast differentiation of human adipose-derived stem cells (hASC), compared to red (660 nm) and near-infrared (NIR, 810 nm). Intracellular calcium was higher after blue/green, and could be inhibited by the ion channel blocker, capsazepine. In the present study we asked what was the effect of these four wavelengths on proliferation of the hASC? When cultured in proliferation medium there was a clear difference between blue/green which inhibited proliferation and red/NIR which stimulated proliferation, all at 3 J/cm2. Blue/green reduced cellular ATP, while red/NIR increased ATP in a biphasic manner. Blue/green produced a bigger increase in intracellular calcium and reactive oxygen species (ROS). Blue/green reduced mitochondrial membrane potential (MMP) and lowered intracellular pH, while red/NIR had the opposite effect. Transient receptor potential vanilloid 1 (TRPV1) ion channel was expressed in hADSC, and the TRPV1 ligand capsaicin (5uM) stimulated proliferation, which could be abrogated by capsazepine. The inhibition of proliferation caused by blue/green could also be abrogated by capsazepine, and by the antioxidant, N-acetylcysteine. The data suggest that blue/green light inhibits proliferation by activating TRPV1, and increasing calcium and ROS.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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