Funded in part by GlaxoSmithKline, GlaxoSmithKline (GSK)
Creatine ( methyl-d3) dilution in urine for estimation of total body skeletal muscle mass: accuracy and variability vs. MRI and DXA
Clark RV, Walker AC, Miller RR, O'Connor-Semmes RL, Ravussin E, Cefalu WT
Journal of applied physiology (Bethesda, Md. : 1985) · 55 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Controlled clinical trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Industry funded
- Company
- GlaxoSmithKline
- Government
- National Institute of Diabetes and Digestive and Kidney Diseases
- Company
- GlaxoSmithKline (GSK)
- Government
- NIDDK NIH HHS
- Grants
- National Institute of Diabetes and Digestive and Kidney Diseases (P30 DK-072476)
Based on 4 listed funder(s).
Publication
- Published
- 2017-08-31 · J Appl Physiol (1985) · vol. 124 · issue 1 · pp. 1–9
- Publisher
- American Physiological Society
- Cited
- 67 citations · more than 88% of similar papers · 2.3× the field average
- References
- 32 works
- Access
- Open access (repository copy)
- Research areas
- Muscle metabolism and nutrition · Nutrition and Health in Aging · Body Composition Measurement Techniques
- Keywords
- Creatine, Creatinine, Urine, Lean body mass, Medicine, Dual-energy X-ray absorptiometry, Internal medicine, Endocrinology, Body weight, Bone mineral
- MeSH
- muscle, skeletal, humans, deuterium, creatine, magnetic resonance imaging, absorptiometry, photon, indicator dilution techniques, body composition, aged, aged, 80 and over, middle aged, female, male
6 authors
From US
- Richard V. Clark · correspondingResearch Triangle Park Foundation; GlaxoSmithKline (United States)
- Ann C. WalkerResearch Triangle Park Foundation; GlaxoSmithKline (United States)
- Ram R. MillerResearch Triangle Park Foundation; GlaxoSmithKline (United States)
- Robin L. O'Connor‐SemmesResearch Triangle Park Foundation; GlaxoSmithKline (United States)
- Éric RavussinPennington Biomedical Research Center
- William T. CefaluPennington Biomedical Research Center
Abstract
A noninvasive method to estimate muscle mass based on creatine ( methyl-d3) (D3-creatine) dilution using fasting morning urine was evaluated for accuracy and variability over a 3- to 4-mo period. Healthy older (67- to 80-yr-old) subjects ( n = 14) with muscle wasting secondary to aging and four patients with chronic disease (58-76 yr old) fasted overnight and then received an oral 30-mg dose of D3-creatine at 8 AM ( day 1). Urine was collected during 4 h of continued fasting and then at consecutive 4- to 8-h intervals through day 5. Assessment was repeated 3-4 mo later in 13 healthy subjects and 1 patient with congestive heart failure. Deuterated and unlabeled creatine and creatinine were measured using liquid chromatography-tandem mass spectrometry. Total body creatine pool size and muscle mass were calculated from D3-creatinine enrichment in urine. Muscle mass was also measured by whole body MRI and 24-h urine creatinine, and lean body mass (LBM) was measured by dual-energy X-ray absorptiometry (DXA). D3-creatinine urinary enrichment from day 5 provided muscle mass estimates that correlated with MRI for all subjects ( r = 0.88, P 3-creatine dilution method, with intrasubject SD for estimated muscle mass of 2.5 kg vs. MRI (0.5 kg) and DXA (0.8 kg). This study supports further clinical validation of the D3-creatine method for estimating muscle mass. NEW & NOTEWORTHY Measurement of creatine ( methyl-d3) (D3-creatine) and D3-creatinine excretion in fasted morning urine samples may be a simple, less costly alternative to MRI or dual-energy X-ray absorptiometry (DXA) to calculate total body muscle mass. The D3-creatine enrichment method provides estimates of muscle mass that correlate well with MRI, and with less bias than DXA. However, intraindividual variability is high with the D3-creatine method. Studies to refine the spot urine sample method for estimation of muscle mass may be warranted.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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