Moderation of hyperuricemia in rats via consuming walnut protein hydrolysate diet and identification of new antihyperuricemic peptides
Li Q, Kang X, Shi C, Li Y, Majumder K, Ning Z, Ren J
Food & function · 69 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2017-09-18 · Food Funct · vol. 9 · issue 1 · pp. 107–116
- Publisher
- Royal Society of Chemistry
- Cited
- 118 citations · more than 90% of similar papers · 2.5× the field average
- References
- 37 works
- Access
- Paywalled
- Research areas
- Gout, Hyperuricemia, Uric Acid · Nuts composition and effects · Alcohol Consumption and Health Effects
- Keywords
- Hydrolysate, Hyperuricemia, Identification (biology), Food science, Chemistry, Moderation, Biochemistry, Uric acid, Biology, Computer science, Botany
- MeSH
- animals, humans, rats, rats, sprague-dawley, juglans, nuts, hyperuricemia, uric acid, xanthine oxidase, peptides, plant proteins, protein hydrolysates, male
7 authors
From CN, US, GB
- Qingyong LiSouth China University of Technology
- Xiaoyan KangSouth China University of Technology
- Chuanchao ShiSouth China University of Technology
- Yujuan LiSouth China University of Technology
- Kaustav MajumderUniversity of Nebraska–Lincoln; Institute of Food Science & Technology
- Zhengxiang NingSouth China University of Technology
Abstract
In this study, walnut meal hydrolysates (WMH) and dephenolized walnut meal hydrolysates (DWMH) were found to effectively decrease the serum uric acid level and protect the renal function in potassium oxonate-induced hyperuricemic rats in vivo as well as inhibit xanthine oxidase in vitro. Two novel antihyperuricemic peptides including WPPKN (640.8 Da) and ADIYTE (710.7 Da) were purified from DWMH via Sephadex G-15 gel filtration and reverse-phase high-performance liquid chromatography and identified by LC-ESI-MS/MS. These peptides displayed high in vitro xanthine oxidase inhibition (XOI) activity with IC50 values of 17.75 ± 0.12 mg mL-1 (WPPKN) and 19.01 ± 0.23 mg mL-1 (ADIYTE). Based on the results of molecular simulation, WPPKN entered into the hydrophobic channel and even obstructed the interaction between xanthine and xanthine oxidase (XO), while ADIYTE was positioned on the surface of the B-chain and blocked the entrance of the substrate to the hydrophobic channel. Therefore, the two peptides are partially responsible for the antihyperuricemic properties of DWMH.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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