Blueberry Metabolites Attenuate Lipotoxicity-Induced Endothelial Dysfunction
Bharat D, Cavalcanti RRM, Petersen C, Begaye N, Cutler BR, Costa MMA, Ramos RKLG, Ferreira MR, Li Y, Bharath LP, Toolson E, Sebahar P, Looper RE, Jalili T, Rajasekaran NS, Jia Z, Symons JD, Anandh Babu PV
Molecular nutrition & food research · 61 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Nonprofit
- American Heart Association
- University or hospital
- University of Utah
- Government
- National Institutes of Health
- Government
- National Institute on Aging
- Government
- National Heart, Lung, and Blood Institute
- Government
- NIA NIH HHS
- Government
- NHLBI NIH HHS
- Grants
- National Institute on Aging (R03AG042860); National Heart, Lung, and Blood Institute (R01HL118067); National Heart, Lung, and Blood Institute (R01HL141540); American Heart Association ((AHA:16GRNT31050004)); National Institute on Aging (R03 AG052848); National Institutes of Health (RO3AGO52848); National Institutes of Health (AG042860); National Institutes of Health (HL118067)
Based on 7 listed funder(s).
Publication
- Published
- 2017-10-12 · Mol Nutr Food Res · vol. 62 · issue 2
- Publisher
- Wiley
- Cited
- 78 citations · more than 94% of similar papers · 3.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 47 works
- Access
- Open access (repository copy)
- Research areas
- Phytochemicals and Antioxidant Activities · Plant Gene Expression Analysis · Biological and pharmacological studies of plants
- Keywords
- Chemistry, Lipotoxicity, Endothelial dysfunction, Nitric oxide, Reactive oxygen species, Biochemistry, Endothelium, Pharmacology, Vanillic acid, Food science, Endocrinology, Medicine, Diabetes mellitus
- MeSH
- aorta, endothelium, vascular, cells, cultured, endothelial cells, animals, mice, inbred c57bl, humans, nitric oxide, reactive oxygen species, anthocyanins, insulin, palmitic acid, gene expression regulation, male, blueberry plants
18 authors
From US
- Divya BharatUniversity of Utah
- Rafaela Ramos Mororo CavalcantiUniversity of Utah
- Chrissa PetersenUniversity of Utah
- Nathan BegayeUniversity of Utah
- Brett Ronald CutlerUniversity of Utah
- Marcella Melo Assis CostaUniversity of Utah
Abstract
Scope
Lipotoxicity-induced endothelial dysfunction is an important vascular complication associated with diabetes. Clinical studies support the vascular benefits of blueberry anthocyanins, but the underlying mechanism is unclear. The hypothesis that metabolites of blueberry anthocyanins attenuate lipotoxicity-induced endothelial dysfunction was tested.
Methods and results
Human aortic endothelial cells (HAECs) were treated for 6 h with either: (i) the parent anthocyanins (malvidin-3-glucoside and cyanidin-3-glucoside); or (ii) the blueberry metabolites (hydroxyhippuric acid, hippuric acid, benzoic acid-4-sulfate, isovanillic acid-3-sulfate, and vanillic acid-4-sulfate), at concentrations known to circulate in humans following blueberry consumption. For the last 5 h HAECs were treated with palmitate or vehicle. HAECs treated with palmitate displayed elevated reactive oxygen species generation, increased mRNA expression of NOX4, chemokines, adhesion molecules, and IκBα, exaggerated monocyte binding, and suppressed nitric oxide production. Of note, the damaging effects of palmitate were ameliorated in HAECs treated with blueberry metabolites but not parent anthocyanins. Further, important translational relevance of these results was provided by our observation that palmitate-induced endothelial dysfunction was lessened in arterial segments that incubated concurrently with blueberry metabolites.
Conclusion
The presented findings indicate that the vascular benefits of blueberry anthocyanins are mediated by their metabolites. Blueberries might complement existing therapies to lessen vascular complications.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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