Comparison of the Micellar Incorporation and the Intestinal Cell Uptake of Cholecalciferol, 25-Hydroxycholecalciferol and 1-α-Hydroxycholecalciferol
Desmarchelier C, Margier M, Prévéraud DP, Nowicki M, Rosilio V, Borel P, Reboul E
Nutrients · 13 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Industry funded
Based on full-text disclosure statement.
Publication
- Published
- 2017-10-23 · Nutrients · vol. 9 · issue 10 · p. 1152
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 25 citations · more than 84% of similar papers · 1.7× the field average
- References
- 29 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Vitamin D Research Studies · Electrolyte and hormonal disorders · Vitamin C and Antioxidants Research
- Keywords
- Cholecalciferol, Vitamin D and neurology, Bioavailability, Internal medicine, Context (archaeology), Calcitriol receptor, Endocrinology, vitamin D deficiency, Vitamin, Chemistry, Medicine, Pharmacology, Biology
- MeSH
- intestines, intestinal mucosa, caco-2 cells, cell membrane, humans, simvastatin, cholecalciferol, hydroxycholecalciferols, calcifediol, membrane transport proteins, membrane proteins, intestinal absorption, micelles, scavenger receptors, class b
7 authors
From FR
- Charles DesmarchelierInserm; Aix-Marseille Université; Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement; Nutrition, Obésité et Risque Thrombotique
- Marielle MargierInserm; Aix-Marseille Université; Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement; Nutrition, Obésité et Risque Thrombotique
- Damien P. Prévéraud
- Marion NowickiInserm; Aix-Marseille Université; Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement; Nutrition, Obésité et Risque Thrombotique
- Véronique RosilioCentre National de la Recherche Scientifique; Université Paris-Saclay; Institut Galien Paris-Saclay
- Patrick BorelInserm; Aix-Marseille Université; Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement; Nutrition, Obésité et Risque Thrombotique
Abstract
In the context of the global prevalence of vitamin D insufficiency, we compared two key determinants of the bioavailability of 3 vitamin D forms with significant biopotencies: cholecalciferol, 25-hydroxycholecalciferol and 1-α-hydroxycholecalciferol. To this aim, we studied their incorporation into synthetic mixed micelles and their uptake by intestinal cells in culture. Our results show that 1-α-hydroxycholecalciferol was significantly more solubilized into mixed micelles compared to the other forms (1.6-fold and 2.9-fold improvement compared to cholecalciferol and 25-hydroxycholecalciferol, respectively). In Caco-2 TC7 cells, the hydroxylated forms were taken up more efficiently than cholecalciferol (p < 0.05), and conversely to cholecalciferol, their uptake was neither SR-BI(Scavenger-Receptor class B type I)- nor NPC1L1 (NPC1 like intracellular cholesterol transporter 1)-dependent. Besides, the apical membrane sodium-bile acid transporter ASBT (Apical Sodium-dependent Bile acid Transporter) was not involved, at least in vitro, in the uptake of any of the three vitamin D forms. Further investigations are needed to identify the uptake pathways of both 1-α-hydroxycholecalciferol and 25-hydroxycholecalciferol. However, considering its high bioavailability, our results suggest the potential interest of using 1-α-hydroxycholecalciferol in the treatment of severe vitamin D deficiency.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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