Anti-inflammatory effects of low-level laser therapy on human periodontal ligament cells: in vitro study
Lee JH, Chiang MH, Chen PH, Ho ML, Lee HE, Wang YH
Lasers in medical science · 44 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Ministry of Science and Technology, Taiwan
- University or hospital
- Kaohsiung Medical University
- Grants
- Ministry of Science and Technology, Taiwan (MOST-104-2314-B-037-059); Ministry of Science and Technology, Taiwan (MOST 104?); Kaohsiung Medical University (KMU-TP103B08); Kaohsiung Medical University (KMU-TP104B11)
Based on 2 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2017-11-07 · Lasers Med Sci · vol. 33 · issue 3 · pp. 469–477
- Publisher
- Springer Science+Business Media
- Cited
- 67 citations · more than 94% of similar papers · 3.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 36 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Laser Applications in Dentistry and Medicine · Oral microbiology and periodontitis research · Connexins and lens biology
- Keywords
- Periodontal fiber, Low level laser therapy, Tumor necrosis factor alpha, Inflammation, Lipopolysaccharide, Intracellular, Cytokine, Chemistry, Panax notoginseng, Interleukin, Pharmacology, Medicine, Immunology, Pathology, Dentistry, Biochemistry, Laser therapy
- MeSH
- periodontal ligament, humans, inflammation, adenine, lipopolysaccharides, nf-kappa b, cyclic amp, anti-inflammatory agents, inflammation mediators, cytokines, cell death, cell survival, transcription, genetic, gene expression regulation, low-level light therapy
6 authors
From TW
- Ji-Hua LeeKaohsiung Medical University
- Min‐Hsuan ChiangKaohsiung Medical University
- Ping‐Ho ChenNational Sun Yat-sen University; Kaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital
- Mei‐Ling HoNational Sun Yat-sen University; Kaohsiung Medical University
- Huey-Er LeeKaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital
- Yan‐Hsiung Wang · correspondingKaohsiung Medical University
Abstract
Periodontal disease is a chronic inflammatory disease that is commonly treated with surgical and nonsurgical techniques. However, both approaches have limitations. Low-level laser therapy (LLLT) has been widely applied in reducing inflammatory reactions, and research indicates that LLLT induces an anti-inflammatory effect that may enhance periodontal disease therapy. The purpose of this study was to investigate the anti-inflammatory effect of LLLT on human periodontal ligament cells (hPDLCs) in an inflammatory environment and aimed to determine the possible mechanism of action. Cells were cultured and treated with or without lipopolysaccharide (LPS) from Porphryromonas gingivalis or Escherichia coli, followed by irradiation with a gallium-aluminum-arsenide (GaAlAs) laser (660 nm) at an energy density of 8 J/cm2. Quantitative real-time polymerase chain reactions were used to assess the expression of pro-inflammatory genes, including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8. The dual-luciferase reporter assay was used to examine nuclear factor-κB (NF-κB) transcriptional activity. An enzyme-linked immunosorbent assay was used to monitor the concentration of intracellular cyclic adenosine monophosphate (cAMP). Both LPS treatments significantly induced the mRNA expression of pro-inflammatory cytokines. However, LLLT inhibited the LPS-induced pro-inflammatory cytokine expression and elevated intracellular levels of cAMP. The LLLT inhibitory effect may function by downregulating NF-κB transcriptional activity and by increasing the intracellular levels of cAMP. LLLT might inhibit LPS-induced inflammation in hPDLCs through cAMP/NF-κB regulation. These results should be further studied to improve periodontal therapy.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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