Cytotoxic Effects and Anti-Angiogenesis Potential of Pistachio (Pistacia vera L.) Hulls against MCF-7 Human Breast Cancer Cells
Seifaddinipour M, Farghadani R, Namvar F, Mohamad J, Abdul Kadir H
Molecules (Basel, Switzerland) · 24 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2018-01-05 · Molecules · vol. 23 · issue 1 · p. 110
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 55 citations · more than 92% of similar papers · 3.3× the field average
- Impact
- Top 10% most cited in its field
- References
- 26 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Nuts composition and effects · Tannin, Tannase and Anticancer Activities · Research in Cotton Cultivation
- Keywords
- Pistacia, MCF-7, Human breast, Angiogenesis, Cytotoxic T cell, Cancer, Breast cancer, Traditional medicine, Biology, Cancer research, Botany, Medicine, Internal medicine, In vitro, Biochemistry
- MeSH
- chorioallantoic membrane, hct116 cells, ht29 cells, chick embryo, animals, humans, pistacia, breast neoplasms, angiogenesis inhibitors, plant extracts, antineoplastic agents, phytogenic, drug screening assays, antitumor, inhibitory concentration 50, apoptosis, cell shape, cell survival, neovascularization, physiologic, female, apoptosis regulatory proteins, mcf-7 cells
5 authors
From MY, IR
- Maryam SeifaddinipourUniversity of Malaya
- Reyhaneh FarghadaniUniversity of Malaya
- Farideh Namvar · correspondingIslamic Azad University, Mashhad
- Jamaludin MohamadUniversity of Malaya
- Habsah Abdul Kadir · correspondingUniversity of Malaya
Abstract
Pistachio (Pistacia vera L.) hulls (PVLH) represents a significant by-product of industrial pistachio processing that contains high amounta of phenolic and flavonoid compounds known to act as antioxidants. The current study was designed to evaluate the anti-tumor and anti-angiogenic potentials of PVLH extracts. The cytotoxic effects of hexane, ethyl acetate, methanol, and water PVLH extracts toward human colon cancer (HT-29 and HCT-116), breast adenocarcinoma (MCF-7), lung adenocarcinoma (H23), liver hepatocellular carcinoma (HepG2), cervical cancer (Ca Ski), and normal fibroblast (BJ-5ta) cells were assessed using a MTT cell viability assay. Apoptosis induction was evaluated through the different nuclear staining assays and confirmed by flow cytometry analysis. Anti-angiogenic activities were also determined using chorioallantoic membrane (CAM) assay. PVLH ethyl acetate extracts (PVLH-EAE) demonstrated a suppressive effect with an IC50 value of 21.20 ± 1.35, 23.00 ± 1.2 and 25.15 ± 1.85 µg/mL against MCF-7, HT-29 and HCT-116, respectively, after 72 h of treatment. Morphological assessment and flow cytometry analysis showed the potential of PVLH-EAE to induce apoptosis. PVLH-EAE at the highest concentration demonstrated significant inhibition of angiogenesis as comparing with control group. Also the expression of Bax increased and the expression of Bcl-2 decreased in treated MCF-7 cells. Thus, the apoptosis induction and angiogenesis potential of PVLH-EAE make it to be the most suitable for further cancer research study to deal with selective antitumor active substances to human cancers especially breast cancer.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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