The standardized Withania somnifera Dunal root extract alters basal and morphine-induced opioid receptor gene expression changes in neuroblastoma cells
Caputi FF, Acquas E, Kasture S, Ruiu S, Candeletti S, Romualdi P
BMC complementary and alternative medicine · 10 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- Università di Bologna
- Government
- Regione Autonoma della Sardegna
- Grants
- Università di Bologna (RFO 2014); Regione Autonoma della Sardegna (CUP B71J11001480002); Regione Autonoma della Sardegna (RAS CRP 26805-CUP B71J11001480002.)
Based on 2 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2018-01-10 · BMC Complement Altern Med · vol. 18 · issue 1 · p. 9
- Publisher
- BioMed Central
- Cited
- 19 citations · more than 90% of similar papers · 3.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 63 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Neuropeptides and Animal Physiology · Pain Mechanisms and Treatments
- Keywords
- Pharmacology, Withania somnifera, Opioid, Morphine, (+)-Naloxone, NOP, Receptor, Opioid receptor, Nociceptin receptor, μ-opioid receptor, Medicine, Gene expression, SH-SY5Y, Chemistry, Opioid peptide, Biology, Internal medicine, Neuroblastoma, Cell culture, Gene, Biochemistry
- MeSH
- cell line, tumor, humans, withania, plant roots, neuroblastoma, receptors, opioid, plant extracts, cell survival, gene expression regulation, neoplastic, real-time polymerase chain reaction
6 authors
From IT
- Francesca Felicia Caputi · correspondingUniversity of Bologna
- Elio AcquasUniversity of Cagliari
- Sanjay Bhaskar Kasture
- Stefania RuiuIstituto di Farmacologia Traslazionale; National Research Council
- Sanzio CandelettiUniversity of Bologna
- Patrizia RomualdiUniversity of Bologna
Abstract
Background
Behavioral studies demonstrated that the administration of Withania somnifera Dunal roots extract (WSE), prolongs morphine-elicited analgesia and reduces the development of tolerance to the morphine's analgesic effect; however, little is known about the underpinning molecular mechanism(s). In order to shed light on this issue in the present paper we explored whether WSE promotes alterations of μ (MOP) and nociceptin (NOP) opioid receptors gene expression in neuroblastoma SH-SY5Y cells.
Methods
A range of WSE concentrations was preliminarily tested to evaluate their effects on cell viability. Subsequently, the effects of 5 h exposure to WSE (0.25, 0.50 and 1.00 mg/ml), applied alone and in combination with morphine or naloxone, on MOP and NOP mRNA levels were investigated.
Results
Data analysis revealed that morphine decreased MOP and NOP receptor gene expression, whereas naloxone elicited their up-regulation. In addition, pre-treatment with naloxone prevented the morphine-elicited gene expression alterations. Interestingly, WSE was able to: a) alter MOP but not NOP gene expression; b) counteract, at its highest concentration, morphine-induced MOP down-regulation, and c) hamper naloxone-induced MOP and NOP up-regulation.
Conclusion
Present in-vitro data disclose novel evidence about the ability of WSE to influence MOP and NOP opioid receptors gene expression in SH-SY5Y cells. Moreover, our findings suggest that the in-vivo modulation of morphine-mediated analgesia by WSE could be related to the hindering of morphine-elicited opioid receptors down-regulation here observed following WSE pre-treatment at its highest concentration.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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