Immunoprofile of α-Gal- and B-antigen-specific responses differentiates red meat-allergic patients from healthy individuals
Apostolovic D, Rodrigues R, Thomas P, Starkhammar M, Hamsten C, van Hage M
Allergy · 47 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Case-control study (indexed by PubMed)
- Studied in
- People
- Main outcome
- Health markers and function
- Intake measured by
- Not stated
Who paid for it
- Funding
- Independent funding
- Nonprofit
- Hjärt-Lungfonden
- University or hospital
- Karolinska Institutet
- Government
- Vetenskapsrådet
- Nonprofit
- Stiftelsen Lars Hiertas Minne
- Nonprofit
- Magnus Bergvalls Stiftelse
- Nonprofit
- Stiftelsen Konung Gustaf V:s 80-årsfond
- Nonprofit
- Astma- och Allergiförbundet
Based on 7 listed funder(s).
Publication
- Published
- 2018-01-10 · Allergy · vol. 73 · issue 7 · pp. 1525–1531
- Publisher
- Wiley
- Cited
- 61 citations · more than 90% of similar papers · 2.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 22 works
- Access
- Paywalled
- Research areas
- Food Allergy and Anaphylaxis Research · Xenotransplantation and immune response · Allergic Rhinitis and Sensitization
- Keywords
- Immunology, Medicine, Antigen, Red meat, Pathology
- MeSH
- basophils, th2 cells, humans, food hypersensitivity, galactose, immunoglobulin e, immunoglobulin g, allergens, abo blood-group system, epitopes, case-control studies, antibody specificity, cross reactions, red meat
6 authors
From SE
- Danijela ApostolovićKarolinska University Hospital; Karolinska Institutet
- Rui Da Silva RodriguesKarolinska University Hospital
- Pierre M. ThomasKarolinska University Hospital; Karolinska Institutet
- Maria StarkhammarStockholm South General Hospital
- Carl HamstenKarolinska University Hospital; Karolinska Institutet
- Marianne van Hage · correspondingKarolinska University Hospital; Karolinska Institutet
Abstract
Background
The galactose-α-1,3-galactose (α-Gal) epitope is involved in red meat allergy. As α-Gal is structurally similar to the blood group B-antigen, we explored the relationship between the immune responses to α-Gal- and the B-antigen in red meat-allergic patients compared to healthy A/O or B blood donors.
Methods
Sera from 51 red meat-allergic patients IgE-positive to α-Gal and 102 healthy blood donors (51 blood group A/O; 51 blood group B) were included. α-Gal- and B-antigen-specific IgE (ImmunoCAP) and IgG/IgG1-4 (ELISA) responses were determined. Basophil activation tests were performed.
Results
Fifteen healthy donors were IgE positive to α-Gal, of which 3 had blood group B. The allergic patients had significantly higher α-Gal IgE levels compared to the healthy donors. The majority of the allergic patients, but none of the healthy donors, had IgE against the B-antigen. Inhibition studies revealed cross-reactivity between α-Gal and the B-antigen. The biological activity of the B-antigen was confirmed by basophil activation tests. Anti-α-Gal IgG1 and IgG4 levels were significantly higher in the patients compared to the healthy donors. Moreover, the IgG response to the B-antigen was comparable between the allergic patients and healthy A/O donors.
Conclusion
Red meat-allergic patients showed significantly higher α-Gal IgE, IgG1 , and IgG4 levels, reflecting a Th2 response, compared to healthy blood donors. Blood group B donors had significantly reduced antibody responses to α-Gal, due to similarities with the B-antigen, resulting in a lower risk of sensitization to α-Gal and development of red meat allergy.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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