Funded in part by U.S. Highbush Blueberry Council, US Highbush Blueberry Council
Dual action of highbush blueberry proanthocyanidins on Aggregatibacter actinomycetemcomitans and the host inflammatory response
Ben Lagha A, LeBel G, Grenier D
BMC complementary and alternative medicine · 30 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Industry funded
- Industry group
- U.S. Highbush Blueberry Council
- University or hospital
- Universitetet i Oslo
- Industry group
- US Highbush Blueberry Council
Based on 3 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2018-01-10 · BMC Complement Altern Med · vol. 18 · issue 1 · p. 10
- Publisher
- BioMed Central
- Cited
- 44 citations · more than 89% of similar papers · 2.4× the field average
- References
- 97 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Bacterial biofilms and quorum sensing · Antimicrobial Resistance in Staphylococcus · Phytochemicals and Antioxidant Activities
- Keywords
- Aggregatibacter actinomycetemcomitans, Viability assay, Microbiology, Biofilm, Grape seed extract, Vaccinium, Porphyromonas gingivalis, Macrophage, Medicine, Biology, Cell, In vitro, Biochemistry, Periodontitis, Bacteria, Pathology, Internal medicine
- MeSH
- u937 cells, tight junctions, macrophages, keratinocytes, humans, periodontitis, proanthocyanidins, nf-kappa b, plant extracts, cytokines, host-pathogen interactions, aggregatibacter actinomycetemcomitans, blueberry plants
3 authors
From CA
- Amel Ben LaghaUniversité Laval
- Geneviève LeBelUniversité Laval
- Daniel Grenier · correspondingNatural Sciences and Engineering Research Council of Canada; Université Laval
Abstract
Background
The highbush blueberry (Vaccinium corymbosum) has a beneficial effect on several aspects of human health. The present study investigated the effects of highbush blueberry proanthocyanidins (PACs) on the virulence properties of Aggregatibacter actinomycetemcomitans and macrophage-associated inflammatory responses.
Methods
PACs were isolated from frozen highbush blueberries using solid-phase chromatography. A microplate dilution assay was performed to determine the effect of highbush blueberry PACs on A. actinomycetemcomitans growth as well as biofilm formation stained with crystal violet. Tight junction integrity of oral keratinocytes was assessed by measuring the transepithelial electrical resistance (TER), while macrophage viability was determined with a colorimetric MTT assay. Pro-inflammatory cytokine and MMP secretion by A. actinomycetemcomitans-stimulated macrophages was quantified by ELISA. The U937-3xκB-LUC monocyte cell line transfected with a luciferase reporter gene was used to monitor NF-κB activation.
Results
Highbush blueberry PACs reduced the growth of A. actinomycetemcomitans and prevented biofilm formation at sub-inhibitory concentrations. The treatment of pre-formed biofilms with the PACs resulted in a loss of bacterial viability. The antibacterial activity of the PACs appeared to involve damage to the bacterial cell membrane. The PACs protected the oral keratinocytes barrier integrity from damage caused by A. actinomycetemcomitans. The PACs also protected macrophages from the deleterious effect of leukotoxin Ltx-A and dose-dependently inhibited the secretion of pro-inflammatory cytokines (IL-1β, IL-6, CXCL8, TNF-α), matrix metalloproteinases (MMP-3, MMP-9), and sTREM-1 by A. actinomycetemcomitans-treated macrophages. The PACs also inhibited the activation of the NF-κB signaling pathway.
Conclusion
The antibacterial and anti-inflammatory properties of highbush blueberry PACs as well as their ability to protect the oral keratinocyte barrier and neutralize leukotoxin activity suggest that they may be promising candidates as novel therapeutic agents.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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