Elucidating the Role of CD84 and AHR in Modulation of LPS-Induced Cytokines Production by Cruciferous Vegetable-Derived Compounds Indole-3-Carbinol and 3,3'-Diindolylmethane
Wang TTY, Pham Q, Kim YS
International journal of molecular sciences · 14 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- U.S. Department of Agriculture
- Government
- National Cancer Institute
- Grants
- U.S. Department of Agriculture (8040-51530-056-00D)
Based on 2 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2018-01-24 · Int J Mol Sci · vol. 19 · issue 2 · p. 339
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 26 citations · more than 72% of similar papers · 0.9× the field average
- References
- 42 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Genomics, phytochemicals, and oxidative stress · Immune Cell Function and Interaction · Immune Response and Inflammation
- Keywords
- Aryl hydrocarbon receptor, Monocyte, Signal transduction, Immune system, Biology, Lipopolysaccharide, Gene knockdown, Cancer research, Cell biology, Chemistry, Immunology, Biochemistry, Transcription factor, Apoptosis
- MeSH
- monocytes, cell line, macrophages, humans, brassicaceae, vegetables, indoles, cytochrome p-450 cyp1a1, lipopolysaccharides, receptors, aryl hydrocarbon, rna, small interfering, cytokines, gene expression, rna interference, cytochrome p-450 cyp1b1, signaling lymphocytic activation molecule family
3 authors
From US
- Thomas J. Wang · correspondingAgricultural Research Service; United States Department of Agriculture; Beltsville Human Nutrition Research Center
- Quynhchi PhamAgricultural Research Service; United States Department of Agriculture; Beltsville Human Nutrition Research Center
- Young Kim · correspondingNational Cancer Institute
Abstract
Modulation of the immune system by cancer protective food bioactives has preventive and therapeutic importance in prostate cancer, but the mechanisms remain largely unclear. The current study tests the hypothesis that the diet-derived cancer protective compounds, indole-3-carbinol (I3C) and 3,3'-diindolylmethane (DIM), affect the tumor microenvironment by regulation of inflammatory responses in monocytes and macrophages. We also ask whether I3C and DIM act through the aryl hydrocarbon (AHR)-dependent pathway or the signaling lymphocyte activation molecule (SLAM) family protein CD84-mediated pathway. The effect of I3C and DIM was examined using the human THP-1 monocytic cell in its un-differentiated (monocyte) and differentiated (macrophage) state. We observed that I3C and DIM inhibited lipopolysaccharide (LPS) induction of IL-1β mRNA and protein in the monocyte form but not the macrophage form of THP-1. Interestingly, CD84 mRNA but not protein was inhibited by I3C and DIM. AHR siRNA knockdown experiments confirmed that the inhibitory effects of I3C and DIM on IL-1β as well as CD84 mRNA are regulated through AHR-mediated pathways. Additionally, the AHR ligand appeared to differentially regulate other LPS-induced cytokines expression. Hence, cross-talk between AHR and inflammation-mediated pathways, but not CD84-mediated pathways, in monocytes but not macrophages may contribute to the modulation of tumor environments by I3C and DIM in prostate cancer.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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