Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells
Pizato N, Luzete BC, Kiffer LFMV, Corrêa LH, de Oliveira Santos I, Assumpção JAF, Ito MK, Magalhães KG
Scientific reports · 174 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2018-01-25 · Sci Rep · vol. 8 · issue 1 · p. 1952
- Publisher
- Nature Portfolio
- Cited
- 232 citations · more than 98% of similar papers · 7.8× the field average
- Impact
- Top 10% most cited in its field
- References
- 73 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Inflammasome and immune disorders · Fatty Acid Research and Health · IL-33, ST2, and ILC Pathways
- Keywords
- Pyroptosis, Programmed cell death, Cancer cell, Breast cancer, Docosahexaenoic acid, Cancer research, Inflammation, Apoptosis, Inflammasome, Cancer, Chemistry, Cell biology, Medicine, Biology, Immunology, Fatty acid, Internal medicine, Biochemistry, Polyunsaturated fatty acid
- MeSH
- cell line, tumor, cell membrane, lysosomes, animals, humans, mice, reactive oxygen species, caspase 1, docosahexaenoic acids, intracellular signaling peptides and proteins, phosphate-binding proteins, hmgb1 protein, neoplasm proteins, signal transduction, cell survival, enzyme activation, interleukin-1beta, inflammasomes, triple negative breast neoplasms, biomarkers, tumor, pyroptosis, gasdermins
8 authors
From BR
- Nathalia PizatoUniversidade de Brasília
- Beatriz Christina LuzeteUniversidade de Brasília
- Larissa Fernanda Melo Vasconcelos KifferUniversidade de Brasília
- Luís Henrique CorrêaUniversidade de Brasília
- Igor de Oliveira SantosUniversidade de Brasília
- José Antônio Fagundes AssumpçãoUniversidade de Brasília
Abstract
The implication of inflammation in pathophysiology of several type of cancers has been under intense investigation. Omega-3 fatty acids can modulate inflammation and present anticancer effects, promoting cancer cell death. Pyroptosis is an inflammation related cell death and so far, the function of docosahexaenoic acid (DHA) in pyroptosis cell death has not been described. This study investigated the role of DHA in triggering pyroptosis activation in breast cancer cells. MDA-MB-231 breast cancer cells were supplemented with DHA and inflammation cell death was analyzed. DHA-treated breast cancer cells triggered increased caspase-1and gasdermin D activation, enhanced IL-1β secretion, translocated HMGB1 towards the cytoplasm, and membrane pore formation when compared to untreated cells, suggesting DHA induces pyroptosis programmed cell death in breast cancer cells. Moreover, caspase-1 inhibitor (YVAD) could protect breast cancer cells from DHA-induced pyroptotic cell death. In addition, membrane pore formation showed to be a lysosomal damage and ROS formation-depended event in breast cancer cells. DHA triggered pyroptosis cell death in MDA-MB-231by activating several pyroptosis markers in these cells. This is the first study that shows the effect of DHA triggering pyroptosis programmed cell death in breast cancer cells and it could improve the understanding of the omega-3 supplementation during breast cancer treatment.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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