Effect of olive oil phenolic compounds on osteoblast differentiation
Melguizo-Rodríguez L, Manzano-Moreno FJ, De Luna-Bertos E, Rivas A, Ramos-Torrecillas J, Ruiz C, García-Martínez O
European journal of clinical investigation · 18 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- Universidad de Granada
- Government
- Junta de Andalucía
- University or hospital
- Department of Nursing (University of Granada)
- Government
- Spanish Ministry of Education
- Government
- BIO277 (Junta de Andalucía)
- Grants
- Universidad de Granada (BIO277); Junta de Andalucía (BIO277)
Based on 5 listed funder(s).
Publication
- Published
- 2018-02-02 · Eur J Clin Invest · vol. 48 · issue 4
- Publisher
- Wiley
- Cited
- 29 citations · more than 81% of similar papers · 1.4× the field average
- References
- 30 works
- Access
- Open access (repository copy) · OTHER-OA
- Research areas
- Bone Metabolism and Diseases · Edible Oils Quality and Analysis · Biological Research and Disease Studies
- Keywords
- Alkaline phosphatase, Osteoblast, Apigenin, Chemistry, Luteolin, Flow cytometry, In vitro, Caffeic acid, In vivo, Biochemistry, Flavonoid, Molecular biology, Biology, Antioxidant, Enzyme, Biotechnology
- MeSH
- cell line, osteoblasts, humans, propionates, caffeic acids, coumaric acids, phenols, apigenin, luteolin, alkaline phosphatase, antigens, cd, flow cytometry, cell differentiation, dose-response relationship, drug, olive oil
7 authors
From ES
- Lucía Melguizo‐RodríguezUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Francisco Javier Manzano‐MorenoUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Elvira De Luna‐BertosUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Ana RivasUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Javier Ramos‐Torrecillas · correspondingUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Concepción RuízUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
Abstract
Background
Osteoporosis is a skeletal disorder characterized by compromised bone strength that predisposes individuals to an increased risk of fracture. Previous in vivo and in vitro studies have reported that phenolic compounds present in extra virgin olive oil have a beneficial effect on osteoblasts in terms of increase cell proliferation. The aim of this study was to determine whether phenolic compounds present in olive oil could modify the expression of cell differentiation markers on osteoblasts.
Study design
An in vitro experimental design was performed using MG-63 osteoblasts cell line.
Methods
MG63 cells were exposed to different doses of luteolin, apigenin, or p-coumaric, caffeic or ferulic acid. Alkaline phosphatase (ALP) was evaluated by spectrophotometry and antigen expression (cluster of differentiation [CD] 54, CD80, CD86 and HLA-DR) by flow cytometry.
Results
At 24 hour, treated groups showed an increased ALP and modulated antigen profile, with respect to the nontreated group.
Conclusion
These results demonstrate that the phenolic compounds studied induce cell maturation in vitro, increasing ALP synthesis and reducing the expression of antigens involved in immune functions of the osteoblast which would improve bone density.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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