Antioxidant activity and apoptotic induction as mechanisms of action of Withania somnifera (Ashwagandha) against a hepatocellular carcinoma cell line
Ahmed W, Mofed D, Zekri AR, El-Sayed N, Rahouma M, Sabet S
The Journal of international medical research · 31 citations
Review labels
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How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2018-02-02 · J Int Med Res · vol. 46 · issue 4 · pp. 1358–1369
- Publisher
- SAGE Publishing
- Cited
- 55 citations · more than 92% of similar papers · 4.0× the field average
- Impact
- Top 10% most cited in its field
- References
- 44 works
- Access
- Open access (journal) · CC-BY-NC
- Research areas
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection · Andrographolide Research and Applications
- Keywords
- Withania somnifera, Antioxidant, Pharmacology, Glutathione, Apoptosis, MTT assay, Fas ligand, Traditional medicine, Medicine, Chemistry, Biochemistry, Programmed cell death, Pathology, Enzyme
- MeSH
- humans, withania, carcinoma, hepatocellular, liver neoplasms, neoplasm proteins, plant extracts, antioxidants, ligands, inhibitory concentration 50, cell cycle, apoptosis, cell proliferation, cell survival, hep g2 cells
6 authors
From EG
- Wafaa Abdallah AhmedCairo University
- Dina MofedCairo University
- Abdel‐Rahman N. ZekriCairo University; Children Cancer Hospital
- Nasr El-SayedSuez Canal University
- Mohamed RahoumaCairo University
- Salwa Sabet · correspondingCairo University
Abstract
Objective To evaluate the antioxidant and apoptotic inductive effects of Withania somnifera (Ashwagandha) leaf extract against a hepatocellular carcinoma cell line. Methods After treating HepG2cells with Ashwagandha water extract (ASH-WX; 6.25 mg/ml-100 mg/ml), cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Antioxidant activities (total antioxidant, glutathione S-transferase and glutathione reductase), Fas-ligand level, tumour necrosis factor-α (TNF-α) level and caspase-3, -8, and -9 activities were measured. Molecular modelling assessed the binding-free energies of Ashwagandha in the cyclin D1 receptor. Results The MTT assay demonstrated increased cytotoxicity following treatment of HepG2 cells with ASH-WX compared with control untreated cells and theIC50was 5% (approximately 5.0 mg/ml). Antioxidant activities, Fas-ligand levels and caspase-3, -8 and -9 activities significantly increased, while TNF-α level significantly decreased following ASH-WX treatment compared with control untreated cells. Molecular docking analysis revealed a good prediction of binding between cyclin D1 and Ashwagandha. There was significant accumulation of ASH-WX-treated HepG2cells in the G0/G1 and G2/M phases compared with the control untreated cells. Conclusion Ashwagandha could be a powerful antioxidant and a promising anticancer agent against HCC.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY-NC).
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