Protective Effects of Blueberry Anthocyanins against H2O2-Induced Oxidative Injuries in Human Retinal Pigment Epithelial Cells
Huang WY, Huang WY, Wu H, Li DJ, Song JF, Xiao YD, Liu CQ, Zhou JZ, Sui ZQ
Journal of agricultural and food chemistry · 73 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Natural Science Foundation of Jiangsu Province
- University or hospital
- Institute Science Research Fund, Jiangsu Academy of Agricultural Sciences
- Government
- Jiangsu Province Agricultural Science and Technology Innovation Fund
- Grants
- Natural Science Foundation of Jiangsu Province (BK20161376)
Based on 3 listed funder(s).
Publication
- Published
- 2018-02-02 · J Agric Food Chem · vol. 66 · issue 7 · pp. 1638–1648
- Publisher
- American Chemical Society
- Cited
- 102 citations · more than 99% of similar papers · 9.6× the field average
- Impact
- Top 10% most cited in its field
- References
- 54 works
- Access
- Paywalled
- Research areas
- Retinal Diseases and Treatments · Phytochemicals and Antioxidant Activities · Neurological Disease Mechanisms and Treatments
- Keywords
- Anthocyanin, Reactive oxygen species, Superoxide dismutase, Malvidin, Oxidative stress, Catalase, Chemistry, Antioxidant, Malondialdehyde, Biochemistry, Glutathione peroxidase, Glutathione, Protein kinase B, Viability assay, Apoptosis, Food science, Cyanidin, Enzyme
- MeSH
- epithelial cells, humans, fruit, hydrogen peroxide, reactive oxygen species, malondialdehyde, anthocyanins, catalase, glutathione peroxidase, superoxide dismutase, protective agents, antioxidants, oxidative stress, retinal pigment epithelium, blueberry plants
8 authors
From CN
- Wuyang HuangJiangsu Academy of Agricultural Sciences
- Han WuJiangsu Academy of Agricultural Sciences
- Dajing LiJiangsu Academy of Agricultural Sciences
- Jiangfeng SongJiangsu Academy of Agricultural Sciences
- Yadong XiaoJiangsu Academy of Agricultural Sciences
- Chunquan LiuJiangsu Academy of Agricultural Sciences
Abstract
Blueberry anthocyanins are considered protective of eye health because of their recognized antioxidant properties. In this study, blueberry anthocyanin extract (BAE), malvidin (Mv), malvidin-3-glucoside (Mv-3-glc), and malvidin-3-galactoside (Mv-3-gal) all reduced H2O2-induced oxidative stress by decreasing the levels of reactive oxygen species and malondialdehyde and increasing the levels of superoxide dismutase, catalase, and glutathione peroxidase in human retinal pigment epithelial cells. BAE and the anthocyanin standards enhanced cell viability from 63.69 ± 3.36 to 86.57 ± 6.92% (BAE), 115.72 ± 23.41% (Mv), 98.15 ± 9.39% (Mv-3-glc), and 127.97 ± 20.09% (Mv-3-gal) and significantly inhibited cell apoptosis (P < 0.01 for all). Mitogen-activated-protein-kinase pathways, including ERK1/2 and p38, were involved in the bioactivities. In addition, the anthocyanins decreased vascular-endothelial-cell-growth-factor levels and activated Akt-signal pathways. These combined results supported the hypothesis that blueberry anthocyanins could inhibit the induction and progression of age-related macular degeneration (AMD) through antioxidant mechanisms.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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