Ashwagandha root extract exerts anti‑inflammatory effects in HaCaT cells by inhibiting the MAPK/NF‑κB pathways and by regulating cytokines
Sikandan A, Shinomiya T, Nagahara Y
International journal of molecular medicine · 42 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2018-04-02 · Int J Mol Med · vol. 42 · issue 1 · pp. 425–434
- Publisher
- Spandidos Publishing
- Cited
- 58 citations · more than 93% of similar papers · 4.3× the field average
- Impact
- Top 10% most cited in its field
- References
- 44 works
- Access
- Free to read
- Research areas
- Phytochemicals and Medicinal Plants · NF-κB Signaling Pathways · Wound Healing and Treatments
- Keywords
- HaCaT, p38 mitogen-activated protein kinases, Proinflammatory cytokine, Tumor necrosis factor alpha, Cytokine, Inflammation, Kinase, Lipopolysaccharide, Downregulation and upregulation, NF-κB, MAPK/ERK pathway, Biology, Pharmacology, Cell biology, Chemistry, Immunology, Biochemistry, In vitro
- MeSH
- cell line, keratinocytes, animals, mice, inbred c57bl, humans, plant roots, inflammation, water, lipopolysaccharides, nf-kappa b, rna, messenger, anti-inflammatory agents, inflammation mediators, plant extracts, cytokines, wound healing, map kinase signaling system, down-regulation, up-regulation, male
3 authors
From JP
- Abudubari SikandanTokyo Denki University
- Takahisa ShinomiyaTokyo Denki University
- Yukitoshi NagaharaTokyo Denki University
Abstract
A paste composed of the boiled leaves and roots of the Ashwagandha plant is used to cure ulcer and swelling in Ayurvedic medicine. However, the effects of the hot water extract of Ashwagandha roots (ASH‑WEX), which is also used in Ayurveda, on skin have not been fully elucidated. Therefore, the present study investigated the anti‑inflammatory activity of ASH‑WEX on skin, by using the human keratinocyte cell line HaCaT. The results indicated that ASH‑WEX significantly inhibited mRNA expression of inflammatory cytokines, including interleukin (IL)‑8, IL‑6, tumor necrosis factor (TNF‑α), IL‑1β and IL‑12, and promoted the mRNA expression of the anti‑inflammatory cytokine transforming growth factor (TGF)‑β1 in HaCaT cells. In addition, ASH‑WEX inhibited the lipopolysaccharide‑induced phosphorylation of p38 and c‑Jun N‑terminal kinase, as well as the nuclear translocation of nuclear factor (NF)‑κB p65. Downregulation of TNF‑α mRNA and upregulation of TGF‑β1 mRNA were also observed in vivo following ASH‑WEX treatment of mouse skin. In conclusion, the present study demonstrated that the anti‑inflammatory effect of ASH‑WEX may be due to its ability to suppress the NF‑κB and mitogen‑activated protein kinase pathways, and to modulate cytokine expression. These results suggest that ASH‑WEX can potentially protect against skin inflammation.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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