Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's)
Naidoo DB, Chuturgoon AA, Phulukdaree A, Guruprasad KP, Satyamoorthy K, Sewram V
BMC complementary and alternative medicine · 16 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Research Foundation
- Government
- South African Medical Research Council
- University or hospital
- Manipal University
- Government
- Medical Research Council
- Government
- Department of Science and Technology, Government of India
Based on 5 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2018-04-10 · BMC Complement Altern Med · vol. 18 · issue 1 · p. 126
- Publisher
- BioMed Central
- Cited
- 31 citations · more than 94% of similar papers · 4.9× the field average
- Impact
- Top 10% most cited in its field
- References
- 81 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Inflammasome and immune disorders · MicroRNA in disease regulation
- Keywords
- Withania somnifera, Peripheral blood mononuclear cell, Viability assay, Tumor necrosis factor alpha, Medicine, Glutathione, Pharmacology, Apoptosis, Immunology, Chemistry, Biochemistry, In vitro, Enzyme, Pathology
- MeSH
- leukocytes, mononuclear, cells, cultured, humans, withania, neoplasms, cachexia, caspases, plant extracts, cytokines, apoptosis, cell survival, thp-1 cells
6 authors
From ZA, IN
- Dhaneshree Bestinee NaidooUniversity of KwaZulu-Natal
- Anil Amichund ChuturgoonUniversity of KwaZulu-Natal
- Alisa PhulukdareeUniversity of KwaZulu-Natal
- Kanive Parashiva GuruprasadManipal Academy of Higher Education
- Kapaettu SatyamoorthyManipal Academy of Higher Education
- Vikash Sewram · correspondingWestern Cape Department of Health; Stellenbosch University
Abstract
Background
Cancer and inflammation are associated with cachexia. Withania somnifera (W. somnifera) possesses antioxidant and anti-inflammatory potential. We investigated the potential of an aqueous extract of the root of W. somnifera (WRE) to modulate cytokines, antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's).
Methods
Cytotoxcity of WRE was determined at 24 and 72 h (h). Oxidant scavenging activity of WRE was evaluated (2, 2-diphenyl-1 picrylhydrazyl assay). Glutathione (GSH) levels, caspase (- 8, - 9, - 3/7) activities and adenosine triphosphate (ATP) levels (Luminometry) were thereafter assayed. Tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 levels were also assessed using enzyme-linked immunosorbant assay.
Results
At 24 h, WRE (0.2-0.4 mg/ml) decreased PBMC viability between 20 and 25%, whereas it increased THP-1 viability between 15 and 23% (p RE increased PBMC viability by 27-39% (0.05, 0.4 mg/ml WRE) whereas decreased THP-1 viability between 9 and 16% (0.05-0.4 mg/ml WRE) (p RE (0.05-0.4 mg/ml, p RE, whereas IL-1β levels were increased by 0.05-0.4 mg/ml WRE (p RE (0.05-0.4 mg/ml) decreased TNF-α, IL-1β and IL-6 levels (p RE (p RE (0.1-0.4 mg/ml) decreased GSH levels in both cell lines (p RE (0.2-0.4 mg/ml) increased PBMC caspase (-8, -3/7) activities whereas WRE (0.05, 0.1, 0.4 mg/ml) increased THP-1 caspase (-9, -3/7) activities (p RE (p RE, whereas decreased by 0.05 and 0.4 mg/ml WRE (72 h, p < 0.0001).
Conclusion
In PBMC's and THP-1 cells, WRE proved to effectively modulate antioxidant activity, inflammatory cytokines and cell death. In THP-1 cells, WRE decreased pro-inflammatory cytokine levels, which may alleviate cancer cachexia and excessive leukaemic cell growth.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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