Photodynamic Effect of Methylene Blue and Low Level Laser Radiation in Head and Neck Squamous Cell Carcinoma Cell Lines
Kofler B, Romani A, Pritz C, Pritz C, Steinbichler TB, Schartinger VH, Riechelmann H, Dudas J
International journal of molecular sciences · 57 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Austrian Science Fund FWF
Based on 1 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2018-04-07 · Int J Mol Sci · vol. 19 · issue 4 · p. 1107
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 83 citations · more than 97% of similar papers · 5.3× the field average
- Impact
- Top 10% most cited in its field
- References
- 79 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Photodynamic Therapy Research Studies · Nanoplatforms for cancer theranostics · Photoacoustic and Ultrasonic Imaging
- Keywords
- Methylene blue, Head and neck squamous-cell carcinoma, Basal cell, Photodynamic therapy, Cell culture, Cancer research, Head and neck, Oncology, Laser, Cell, Medicine, Pathology, Internal medicine, Head and neck cancer, Chemistry, Radiation therapy, Optics, Biology, Surgery, Physics, Biochemistry
- MeSH
- cell line, tumor, humans, carcinoma, squamous cell, mouth neoplasms, methylene blue, photosensitizing agents, photochemotherapy, dose-response relationship, radiation, cell proliferation, cell survival, dose-response relationship, drug
7 authors
From AT
- Barbara Kofler · correspondingInnsbruck Medical University; Universität Innsbruck; Tirol Kliniken
- Angela RomaniInnsbruck Medical University; Universität Innsbruck; Tirol Kliniken
- Christian Oliver PritzInnsbruck Medical University; Universität Innsbruck
- Teresa Bernadette SteinbichlerInnsbruck Medical University; Universität Innsbruck; Tirol Kliniken
- Volker Hans SchartingerInnsbruck Medical University; Universität Innsbruck
- Herbert RiechelmannInnsbruck Medical University; Universität Innsbruck
Abstract
Photodynamic therapy (PDT) is suggested to have an impact on the treatment of early stage head and neck cancers (HNSCC). We investigated the effect of PDT with methylene blue (MB) and a diode laser (660 nm) as the laser source on HNSCC cell lines as an in vitro model of surface oral squamous cell carcinoma. Cell-cultures were exposed to 160 µM MB for 4 min and to laser light for 8 min. Viability was proven via cell viability assay and clonogenic survival via clone counting assay. The combination of MB and diode laser evidenced high efficient loss of cell viability by 5% of the control, while treatment with the same concentration of MB for 4 min alone showed a viability of 46% of the control. In both SCC-25 and Detroit 562 HNSCC cells, MB combined with the laser allowed a significant abrogation of clonogenic growth (p < 0.01), especially in the case of Detroit 562 cells less than 1% of the suspension plated cells were able to grow tumor cell nests. Multiresistant (Detroit 562) HNSCC cells expressing cancer stem cell markers are sensitive to MB/red laser combined PDT.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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