Synergism between luteolin and sulforaphane in anti-inflammation
Rakariyatham K, Wu X, Tang Z, Han Y, Wang Q, Xiao H
Food & function · 37 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- U.S. Department of Agriculture
- Government
- National Natural Science Foundation of China
- Grants
- U.S. Department of Agriculture (2014-67021); National Natural Science Foundation of China (31671858); U.S. Department of Agriculture (MAS00492); U.S. Department of Agriculture (MAS00450)
Based on 2 listed funder(s).
Publication
- Published
- 2018-01-01 · Food Funct · vol. 9 · issue 10 · pp. 5115–5123
- Publisher
- Royal Society of Chemistry
- Cited
- 52 citations · more than 85% of similar papers · 1.8× the field average
- References
- 37 works
- Access
- Paywalled
- Research areas
- Genomics, phytochemicals, and oxidative stress · Immune Cell Function and Interaction · Reproductive System and Pregnancy
- Keywords
- Sulforaphane, Luteolin, Inflammation, Lipopolysaccharide, Chemistry, Pharmacology, Biochemistry, Medicine, Immunology, Flavonoid, Antioxidant
- MeSH
- macrophages, animals, humans, mice, inflammation, reactive oxygen species, isothiocyanates, sulfoxides, luteolin, nf-kappa b, anti-inflammatory agents, drug synergism, nf-e2-related factor 2, cyclooxygenase 2, heme oxygenase-1, interleukin-1beta, raw 264.7 cells
6 authors
From US, CN, GB
- Kanyasiri RakariyathamAmherst College; University of Massachusetts Amherst
- Xian WuAmherst College; University of Massachusetts Amherst
- Zhonghai TangChangsha University; Institute of Food Science & Technology; Hunan Agricultural University
- Yanhui HanAmherst College; University of Massachusetts Amherst
- Qi WangAmherst College; University of Massachusetts Amherst
- Hang Xiao · correspondingChangsha University; University of Massachusetts Amherst; Institute of Food Science & Technology; Hunan Agricultural University
Abstract
Luteolin and sulforaphane are well-known food bioactives with anti-inflammatory properties. Herein, we determined their combinational effects in inhibiting inflammation in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Both luteolin and sulforaphane showed dose-dependent inhibition on LPS-induced production of nitric oxide (NO) in the macrophages. The combined treatments led to a stronger inhibition on NO production compared to the singular treatments. Isobologram analysis confirmed that the combined treatments produced a synergy. Western blotting and ELISA showed that the combined treatment reduced the expression levels of pro-inflammatory proteins involving NF-κB pathway, and STAT3 activation, which regulated expression of other inflammatory proteins such as iNOS, COX-2, IL-6, and IL-1β. Moreover, the combination treatments reduced reactive oxygen species in cells and increased the expression of Nrf2 and HO-1, which are cellular antioxidant proteins. In conclusion, our findings support the notion that certain bioactive food components may act synergistically to produce enhanced health effects such as anti-inflammation.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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