Walnut ( Juglans regia) Peptides Reverse Sleep Deprivation-Induced Memory Impairment in Rat via Alleviating Oxidative Stress
Wang S, Su G, Zhang Q, Zhao T, Liu Y, Zheng L, Zhao M
Journal of agricultural and food chemistry · 58 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Government
- Ministry of Science and Technology of the People's Republic of China
- Government
- Government of Guangdong Province
- Government
- Ministry of Human Resources and Social Security
- Government
- Guangzhou Science and Technology Plan Projects
- Government
- Ministry of Science and Technology of the People's Republic of China
- Grants
- Government of Guangdong Province (2016TQ03N728); Ministry of Human Resources and Social Security (BX201700081); Ministry of Science and Technology of the People's Republic of China (2017YFD0400201)
Based on 5 listed funder(s).
Publication
- Published
- 2018-09-18 · J Agric Food Chem · vol. 66 · issue 40 · pp. 10617–10627
- Publisher
- American Chemical Society
- Cited
- 91 citations · more than 87% of similar papers · 1.9× the field average
- References
- 48 works
- Access
- Paywalled
- Research areas
- Protein Hydrolysis and Bioactive Peptides · Biochemical effects in animals · Fatty Acid Research and Health
- Keywords
- Neuroprotection, Oxidative stress, Superoxide dismutase, Malondialdehyde, Glutathione peroxidase, Pharmacology, Chemistry, Antioxidant, Glutamate receptor, Biochemistry, Reactive oxygen species, Glutathione, Viability assay, Apoptosis, Biology, Enzyme
- MeSH
- pc12 cells, animals, humans, rats, juglans, sleep deprivation, reactive oxygen species, malondialdehyde, catalase, glutathione peroxidase, superoxide dismutase, peptides, neuroprotective agents, memory, apoptosis, oxidative stress, female, male, membrane potential, mitochondrial
7 authors
From CN
- Shuguang WangSouth China University of Technology
- Guowan SuSouth China University of Technology
- Qi ZhangSouth China University of Technology
- Tiantian ZhaoSouth China University of Technology
- Yang LiuSouth China University of Technology
- Lin Ping Zheng · correspondingSouth China University of Technology
Abstract
The aim of this study was to determine the neuroprotective effects of walnut protein hydrolysates (WPH) against memory deficits induced by sleep deprivation (SD) in rat and further to identify and characterize the potent neuroprotective peptides against glutamate-induced apoptosis in PC12 cells. Results showed that a remarkable amelioration effect on behavioral performance in Morris water maze test was observed for WPH and its low molecular weight fraction WPHL, especially for WPHL. Additionally, a reduction of antioxidant defense (catalase, glutathione peroxidase (GSH-px), and superoxide dismutase (SOD)) and an increase of malondialdehyde content induced by SD were normalized in brain of rat after oral administration of WPH and WPHL. Then three neuroprotective peptides including GGW, VYY, and LLPF were identified from WPHL, which could protect PC12 cells against glutamate-induced apoptosis with relative cell viability of 78.29 ± 3.09%, 80.65 ± 1.74%, and 83.97 ± 3.06%, respectively, versus glutamate group 48.61 ± 3.99%. The possible mechanism underlying their protective effects of GGW and VYY could be related to their strong radical scavenging activity as well as their ability to reduce reactive oxygen species production and the depletion of SOD and GSH-px in PC12 cells. Notably, the marked neuroprotective effects of LLPF, which did not show obvious free-radical scavenging activity in vitro, could be attributed to its strong effects on inhibiting Ca2+ influx and mitochondrial membrane potential collapse. Additionally, all these peptides could regulate the expression of apoptosis-related proteins (Bax and Bcl-2). Therefore, walnut peptides might be regarded as the potential nutraceuticals against neurodegenerative disorders associated with memory deficits.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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