An integrated framework using high-dimensional mass cytometry and fluorescent flow cytometry identifies discrete B cell subsets in patients with red meat allergy
Cox KM, Commins SP, Capaldo BJ, Workman LJ, Platts-Mills TAE, Amir ED, Lannigan JA, Schuyler AJ, Erickson LD
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 14 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Case-control study (indexed by PubMed)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Not stated
Who paid for it
- Funding
- Independent funding
- University or hospital
- University of Virginia
- Government
- National Institutes of Health
- Government
- National Institute of Allergy and Infectious Diseases
- Government
- NIAID NIH HHS
- Grants
- National Institute of Allergy and Infectious Diseases (R56 AI113095); National Institute of Allergy and Infectious Diseases (R37 AI020565); National Institute of Allergy and Infectious Diseases (R21 AI124490); National Institute of Allergy and Infectious Diseases (R01AI-020565); National Institute of Allergy and Infectious Diseases (R01 AI135049); National Institutes of Health (R21 AI124490); National Institutes of Health (R01-AI20565)
Based on 4 listed funder(s).
Publication
- Published
- 2018-12-02 · Clin Exp Allergy · vol. 49 · issue 5 · pp. 615–625
- Publisher
- Wiley
- Cited
- 18 citations · more than 73% of similar papers · 1.0× the field average
- References
- 54 works
- Access
- Open access (repository copy)
- Research areas
- Food Allergy and Anaphylaxis Research · T-cell and B-cell Immunology · Celiac Disease Research and Management
- Keywords
- Flow cytometry, ELISPOT, Immunoglobulin D, Immunoglobulin E, Immunology, Peripheral blood mononuclear cell, Food allergy, Cytometry, Allergy, B cell, Mass cytometry, Biology, Antibody, T cell, Phenotype, In vitro, Immune system, Genetics, Gene
- MeSH
- b-lymphocyte subsets, leukocytes, mononuclear, humans, food hypersensitivity, immunoglobulin e, lymphocyte count, flow cytometry, cluster analysis, case-control studies, immunophenotyping, adult, aged, middle aged, disease management, female, male, young adult, biomarkers, red meat
9 authors
From US
- Kelly M. CoxCarter Center; University of Virginia
- Scott P. ComminsUniversity of North Carolina at Chapel Hill
- Brian J. CapaldoUniversity of Virginia
- Lisa J. WorkmanUniversity of Virginia
- Thomas A.E. Platts‐MillsCarter Center; University of Virginia
- El-ad David Amir
Abstract
Background
B cells play a critical role in the development and maintenance of food allergy by producing allergen-specific IgE. Despite the importance of B cells in IgE-mediated food allergy, the identity of sIgE-producing human B cells and how IgE is regulated are poorly understood.
Objective
To identify the immunophenotypes of circulating B cells associated with the production of galactose-alpha-1,3-galactose-specific IgE production in patients with red meat allergy.
Methods
B cells in PBMC samples obtained from 19 adults with physician-diagnosed red meat allergy and 20 non-meat allergic healthy controls were assessed by mass cytometry along with a bioinformatics analysis pipeline to identify discrete B cell phenotypes that associated with serum sIgE. Fluorescent flow cytometry was then applied to sort purify discrete B cell subsets, and B cells were functionally evaluated on an individual cell level for the production of sIgE by ELISPOT.
Results
Discrete B cell phenotypes abundant in meat allergic subjects compared to non-meat allergic controls were found in peripheral blood that do not share typical characteristics of classical isotype-switched memory B cells that express high levels of CD27. These B cell subsets shared higher IgD and lower IgM expression levels coupled with CXCR4, CCR6 and CD25 expression. In vitro polyclonal stimulation of purified B cell subsets from meat allergic subjects demonstrated that these subsets were enriched for cells induced to secrete sIgE.
Conclusions and clinical relevance
Circulating B cells display increased abundance of discrete B cell subsets in meat allergic subjects. This observation, coupled with the capacity of individual B cell subsets to produce sIgE following activation, implicates these novel B cell phenotypes in promoting IgE in meat allergy.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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