Photobiomodulation at Multiple Wavelengths Differentially Modulates Oxidative Stress In Vitro and In Vivo
Rupel K, Zupin L, Colliva A, Kamada A, Poropat A, Ottaviani G, Gobbo M, Fanfoni L, Gratton R, Santoro M, Di Lenarda R, Biasotto M, Zacchigna S
Oxidative medicine and cellular longevity · 54 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Controlled clinical trial (indexed by PubMed)
- Studied in
- People, plus animal or lab work
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- International Centre for Genetic Engineering and Biotechnology
- Nonprofit
- Associazione Italiana per la Ricerca sul Cancro
- Authors
- At least one author declares a financial tie to industry
- Grants
- Associazione Italiana per la Ricerca sul Cancro (IG2016); Associazione Italiana per la Ricerca sul Cancro (AIRC-IG #2016-19032); Associazione Italiana per la Ricerca sul Cancro (2016 19032); Associazione Italiana per la Ricerca sul Cancro (IG 2016 19032); Associazione Italiana per la Ricerca sul Cancro (19032)
Based on 2 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2018-01-01 · Oxid Med Cell Longev · vol. 2018 · issue 1 · p. 6510159
- Publisher
- Hindawi Publishing Corporation
- Cited
- 79 citations · more than 95% of similar papers · 4.2× the field average
- Impact
- Top 10% most cited in its field
- References
- 39 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Laser Applications in Dentistry and Medicine · Oral health in cancer treatment · Photodynamic Therapy Research Studies
- Keywords
- Oxidative stress, Reactive oxygen species, In vivo, Mucositis, In vitro, Viability assay, Inflammation, Antioxidant, Cell biology, Chemistry, Biology, Cancer research, Immunology, Medicine, Biochemistry, Toxicity, Internal medicine, Biotechnology
- MeSH
- neutrophils, keratinocytes, humans, stomatitis, oxidation-reduction, oxidative stress, adult, aged, aged, 80 and over, middle aged, female, male, lasers, semiconductor, low-level light therapy
13 authors
From IT, BR
- Katia RupelUniversity of Trieste
- Luisa ZupinUniversity of Trieste
- Andrea CollivaInternational Centre for Genetic Engineering and Biotechnology
- Anselmo Jiro KamadaUniversidade Federal de Pernambuco
- Augusto PoropatUniversity of Trieste
- Giulia OttavianiUniversity of Trieste
Abstract
Photobiomodulation (PBM) is emerging as an effective strategy for the management of multiple inflammatory conditions, including oral mucositis (OM) in cancer patients who receive chemotherapy or radiotherapy. Still, the poor understanding of the mechanisms by which the light interacts with biological tissues and the heterogeneity of light sources and protocols employed worldwide significantly limits its applicability. Reactive oxygen species (ROS) are massively generated during the early phases of OM and play a major role in the pathogenesis of inflammation in general. Here, we report the results of a clinical and experimental study, aimed at evaluating the effect of laser light at different wavelengths on oxidative stress in vivo in oncologic patients suffering from OM and in vitro in two cell types abundantly present within the inflamed oral mucosa, neutrophil polymorphonuclear (PMN) granulocytes, and keratinocytes. In addition to standard ROS detection methods, we exploited a roGFP2-Orp1 genetically encoded sensor, allowing specific, quantitative, and dynamic imaging of redox events in living cells in response to oxidative stress and PBM. We found that the various wavelengths differentially modulate ROS production. In particular, the 660 nm laser light increases ROS production when applied either before or after an oxidative stimulus. In contrast, the 970 nm laser light exerted a moderate antioxidant activity both in the saliva of OM patients and in both cell types. The most marked reduction in the levels of ROS was detected in cells exposed either to the 800 nm laser light or to the combination of the three wavelengths. Overall, our study demonstrates that PBM exerts different effects on the redox state of both PMNs and keratinocytes depending on the used wavelength and prompts the validation of a multiwavelength protocol in the clinical settings.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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