Toxic doses of caffeine are needed to increase skeletal muscle contractility
Neyroud D, Cheng AJ, Donnelly C, Bourdillon N, Gassner AL, Geiser L, Rudaz S, Kayser B, Westerblad H, Place N
American journal of physiology. Cell physiology · 27 citations
How it was studied
- Design
- Randomized controlled trial (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Government
- Vetenskapsrådet
- University or hospital
- Centrum för idrottsforskning
- University or hospital
- Université de Lausanne
- Government
- Swedish Research Council for Sport Science
- Government
- Swedish Research Council
- University or hospital
- University of Lausanne
- Grants
- Vetenskapsrådet (K2014‐52X‐10842‐21‐5); Centrum för idrottsforskning (FO2018-0019); Centrum för idrottsforskning (FO2016-033)
Based on 6 listed funder(s).
Publication
- Published
- 2018-12-19 · Am J Physiol Cell Physiol · vol. 316 · issue 2 · pp. C246–C251
- Publisher
- American Physical Society
- Cited
- 41 citations · more than 86% of similar papers · 1.9× the field average
- References
- 35 works
- Access
- Free to read
- Research areas
- Coffee research and impacts · Exercise and Physiological Responses · Cardiovascular and exercise physiology
- Keywords
- Caffeine, Contractility, Chemistry, Endocrinology, Internal medicine, Skeletal muscle, Muscle contraction, Medicine
- MeSH
- muscle, skeletal, animals, mice, inbred c57bl, humans, mice, caffeine, electromyography, organ culture techniques, muscle contraction, dose-response relationship, drug, adult, female, male, young adult
10 authors
From CH, SE
- Daria Neyroud · correspondingUniversity of Lausanne
- Arthur J. ChengKarolinska Institutet
- Chris DonnellyUniversity of Lausanne
- Nicolas BourdillonUniversity of Lausanne
- Anne‐Laure GassnerUniversity of Geneva; University of Lausanne
- Laurent GeiserUniversity of Geneva; University of Lausanne
Abstract
Discrepant results have been reported regarding an intramuscular mechanism underlying the ergogenic effect of caffeine on neuromuscular function in humans. Here, we reevaluated the effect of caffeine on muscular force production in humans and combined this with measurements of the caffeine dose-response relationship on force and cytosolic free [Ca2+] ([Ca2+]i) in isolated mouse muscle fibers. Twenty-one healthy and physically active men (29 ± 9 yr, 178 ± 6 cm, 73 ± 10 kg, mean ± SD) took part in the present study. Nine participants were involved in two experimental sessions during which supramaximal single and paired electrical stimulations (at 10 and 100 Hz) were applied to the femoral nerve to record evoked forces. Evoked forces were recorded before and 1 h after ingestion of 1) 6 mg caffeine/kg body mass or 2) placebo. Caffeine plasma concentration was measured in 12 participants. In addition, submaximal tetanic force and [Ca2+]i were measured in single mouse flexor digitorum brevis (FDB) muscle fibers exposed to 100 nM up to 5 mM caffeine. Six milligrams of caffeine per kilogram body mass (plasma concentration ~40 µM) did not increase electrically evoked forces in humans. In superfused FDB single fibers, millimolar caffeine concentrations (i.e., 15- to 35-fold above usual concentrations observed in humans) were required to increase tetanic force and [Ca2+]i. Our results suggest that toxic doses of caffeine are required to increase muscle contractility, questioning the purported intramuscular ergogenic effect of caffeine in humans.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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