Blueberry Malvidin-3-galactoside Suppresses Hepatocellular Carcinoma by Regulating Apoptosis, Proliferation, and Metastasis Pathways In Vivo and In Vitro
Wang Y, Lin J, Tian J, Si X, Jiao X, Zhang W, Gong E, Li B
Journal of agricultural and food chemistry · 51 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Natural Science Foundation of China
- Government
- Shenyang Science and Technology Bureau
- Government
- Liaoning Province
- Grants
- National Natural Science Foundation of China (31671863); Shenyang Science and Technology Bureau (RC170247)
Based on 3 listed funder(s).
Publication
- Published
- 2018-12-26 · J Agric Food Chem · vol. 67 · issue 2 · pp. 625–636
- Publisher
- American Chemical Society
- Cited
- 79 citations · more than 95% of similar papers · 3.5× the field average
- Impact
- Top 10% most cited in its field
- References
- 44 works
- Access
- Paywalled
- Research areas
- Phytochemicals and Antioxidant Activities · Plant biochemistry and biosynthesis · Antioxidant Activity and Oxidative Stress
- Keywords
- Apoptosis, Cancer research, Protein kinase B, PTEN, Cyclin D1, Tensin, PI3K/AKT/mTOR pathway, Biology, Chemistry, Molecular biology, Biochemistry, Cell cycle
- MeSH
- cell line, tumor, animals, mice, inbred c57bl, humans, mice, fruit, carcinoma, hepatocellular, liver neoplasms, neoplasm metastasis, anthocyanins, plant extracts, signal transduction, apoptosis, cell proliferation, cell movement, male, pten phosphohydrolase, matrix metalloproteinase 2, matrix metalloproteinase 9, caspase 3, hep g2 cells, blueberry plants
8 authors
From CN
- Yuehua WangShenyang Agricultural University
- Jie LinShenyang Agricultural University
- Jinlong TianShenyang Agricultural University
- Xu SiShenyang Agricultural University
- Xinyao JiaoShenyang Agricultural University
- Weijia ZhangShenyang Agricultural University
Abstract
Anthocyanin, a natural antioxidant, is reported to have cytotoxicity against cancer cells; however, the mechanism remains unclear. The aim of the present study was to investigate the mechanism by which malvidin-3-galactoside (M3G), the prominent anthocyanin in blueberry, suppresses the development of hepatocellular carcinoma. In vitro, M3G suppressed the proliferation, polarization, migration, and invasion activities of HepG2 cells by regulating the protein expression of cyclin D1, cyclin B, cyclin E, caspase-3, cleaved caspase-3, Bax, p-JNK, and p-p38, activating phosphatase and tensin homologue deleted on chromosome 10 (PTEN), accompanied by a decrease in the p-AKT level, and lowering the protein expression levels of MMP-2 and MMP-9. In vivo, M3G promoted the apoptosis of liver tumor cells, as determined by immunohistochemistry (cleaved caspase-3, Ki-67, PTEN, and p-AKT), a terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and hematoxylin-eosin staining. Overall, these results suggest that M3G, as an adjuvant ingredient or nutritional supplement, may be beneficial for liver cancer prevention and the modulatory mechanism seems to be associated with inhibition of proliferation, apoptosis, migration, and invasion-related pathways.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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