Fish Oil Derived Omega 3 Fatty Acids Suppress Adipose NLRP3 Inflammasome Signaling in Human Obesity
Lee KR, Midgette Y, Shah R
Journal of the Endocrine Society · 43 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Institute of Diabetes and Digestive and Kidney Diseases
- Government
- National Center for Advancing Translational Sciences
- Government
- NIDDK NIH HHS
- Government
- NCATS NIH HHS
- Grants
- National Institute of Diabetes and Digestive and Kidney Diseases (P30 DK-019525); National Center for Advancing Translational Sciences (UL1-TR-001878); National Institute of Diabetes and Digestive and Kidney Diseases (K23 DK095913); National Institute of Diabetes and Digestive and Kidney Diseases (DK095913)
Based on 4 listed funder(s).
Publication
- Published
- 2018-12-24 · J Endocr Soc · vol. 3 · issue 3 · pp. 504–515
- Publisher
- Endocrine Society
- Cited
- 56 citations · more than 94% of similar papers · 3.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 60 works
- Access
- Open access (journal) · CC-BY-NC-ND
- Research areas
- Fatty Acid Research and Health · Adipokines, Inflammation, and Metabolic Diseases · Adipose Tissue and Metabolism
- Keywords
- Fish oil, Inflammasome, Adipose tissue, Obesity, Fish <Actinopterygii>, Omega, Chemistry, Endocrinology, Internal medicine, Biochemistry, Medicine, Biology, Fishery, Receptor, Physics
3 authors
From US
- Kailey Roberts LeeChildren's Hospital of Philadelphia
- Yasmeen A. MidgetteChildren's Hospital of Philadelphia
- Rachana D. Shah · correspondingChildren's Hospital of Philadelphia
Abstract
Context
The NRLP3 inflammasome is a multiprotein danger-sensing complex that serves as a critical link between obesity-related adipose inflammation and insulin resistance and has been shown in animal models to be inhibited by fish oil-derived long chain omega-3 polyunsaturated fatty acids (n-3 PUFA).
Objective
We conducted a clinical trial and in vitro experiments to test our hypothesis that n-3 PUFA suppress NLRP3 inflammasome in human obesity through downregulation of inflammasome gene expression in adipocytes and macrophages.
Design
Placebo-controlled clinical trial and in vitro coculture experiments with primary human adipocytes (from biopsy specimens) and human THP-1 monocyte-derived macrophages treated with eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA) vs vehicle control.
Setting
General community, research laboratory.
Patients and other participants
Obese (body mass index ≥ 30 kg/m2), nondiabetic males and females age 18 to 50. N = 25.
Interventions
Clinical trial: Eight-week treatment with 4 g Lovaza (EPA and DHA) or placebo. Cells culture: EPA and/or DHA at 100 µg/mL or vehicle control in culture medium.
Main outcome measures
Adipose tissue or adipocyte/macrophage mRNA expression of IL-1β and IL-18 and circulating IL-18 levels.
Results
Treatment of obese human subjects with fish oil supplements reduced expression of adipose inflammatory genes including inflammasome-associated IL-18 and IL-1β and circulating IL-18 levels. Both EPA and DHA reduced inflammasome gene expression in obese human adipose and human adipocyte and macrophages.
Conclusions
N-3 PUFA reduce NLRP3 inflammasome in human adipose through downregulation of gene expression in adipocytes and monocytes/macrophages and has potential as nutritional therapeutic agent in prevention of obesity-related inflammation.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY-NC-ND).
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