Antioxidative Effects and Mechanism Study of Bioactive Peptides from Defatted Walnut ( Juglans regia L.) Meal Hydrolysate
Sheng J, Yang X, Chen J, Peng T, Yin X, Liu W, Liang M, Wan J, Yang X
Journal of agricultural and food chemistry · 57 citations
Review labels
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How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Natural Science Foundation of China
- Government
- China Postdoctoral Science Foundation
- Grants
- National Natural Science Foundation of China (81703443); China Postdoctoral Science Foundation (2017M622461)
Based on 2 listed funder(s).
Publication
- Published
- 2019-02-28 · J Agric Food Chem · vol. 67 · issue 12 · pp. 3305–3312
- Publisher
- American Chemical Society
- Cited
- 94 citations · more than 94% of similar papers · 3.6× the field average
- Impact
- Top 10% most cited in its field
- References
- 33 works
- Access
- Paywalled
- Research areas
- Protein Hydrolysis and Bioactive Peptides · Muscle metabolism and nutrition · Nuts composition and effects
- Keywords
- Juglans, Hydrolysate, Food science, Chemistry, Juglandaceae, Meal, Mechanism (biology), Traditional medicine, Biochemistry, Chromatography, Medicine, Hydrolysis
- MeSH
- neurons, cell line, humans, juglans, nuts, reactive oxygen species, peptides, plant proteins, protein hydrolysates, plant extracts, antioxidants, oxidative stress
9 authors
From CN
- Jianyong ShengHuazhong University of Science and Technology
- Xiaoyu YangHuazhong University of Science and Technology
- Jitang ChenHuazhong University of Science and Technology
- Tianhao PengHuazhong University of Science and Technology
- Xiquan YinKey Laboratory of Guangdong Province; Infinitus (China); Huazhong University of Science and Technology
- Wei LiuHuazhong University of Science and Technology
Abstract
The peptide components of defatted walnut ( Juglans regia L.) meal hydrolysate (DWMH) remain unclear, hindering the investigation of biological mechanisms and exploitation of bioactive peptides. The present study aims to identify the peptide composition of DWMH, followed by to evaluate in vitro antioxidant effects of selected peptides and investigate mechanisms of antioxidative effect. First, more than 1 000 peptides were identified by de novo sequencing in DWMH. Subsequently, a scoring method was established to select promising bioactive peptides by structure based screening. Eight brand new peptides were selected due to their highest scores in two different batches of DWMH. All of them showed potent in vitro antioxidant effects on H2O2-injured nerve cells. Four of them even possessed significantly stronger effects than DWMH, making the selected bioactive peptides useful for further research as new bioactive entities. Two mechanisms of hydroxyl radical scavenging and ROS reduction were involved in their antioxidative effects at different degrees. The results showed peptides possessing similar capacity of hydroxyl radical scavenging or ROS reduction may have significantly different in vitro antioxidative effects. Therefore, comprehensive consideration of different antioxidative mechanisms were suggested in selecting antioxidative peptides from DWMH.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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