In Vitro and in Vivo Studies on the Angiotensin-Converting Enzyme Inhibitory Activity Peptides Isolated from Broccoli Protein Hydrolysate
Dang Y, Zhou T, Hao L, Cao J, Sun Y, Pan D
Journal of agricultural and food chemistry · 49 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Government
- National Natural Science Foundation of China
- Government
- Science and Technology Department of Zhejiang Province
- Nonprofit
- K.C. Wong Magna Fund
- Grants
- Science and Technology Department of Zhejiang Province (2018C02051); Science and Technology Department of Zhejiang Province (2014C02023); National Natural Science Foundation of China (31771945)
Based on 3 listed funder(s).
Publication
- Published
- 2019-06-03 · J Agric Food Chem · vol. 67 · issue 24 · pp. 6757–6764
- Publisher
- American Chemical Society
- Cited
- 98 citations · more than 94% of similar papers · 3.6× the field average
- Impact
- Top 10% most cited in its field
- References
- 32 works
- Access
- Paywalled
- Research areas
- Protein Hydrolysis and Bioactive Peptides · Insect Utilization and Effects · Biochemical effects in animals
- Keywords
- Hydrolysate, In vivo, Peptide, IC50, Chemistry, Inhibitory postsynaptic potential, In vitro, Pharmacology, Renin–angiotensin system, Digestion (alchemy), Enzyme, Angiotensin-converting enzyme, Biochemistry, Pepsin, Endocrinology, Biology, Hydrolysis, Chromatography
- MeSH
- animals, rats, inbred shr, humans, rats, brassica, hypertension, peptidyl-dipeptidase a, peptides, plant proteins, protein hydrolysates, antihypertensive agents, angiotensin-converting enzyme inhibitors, peptide mapping, administration, oral, blood pressure, female
6 authors
From CN
- Yali DangNingbo University
- Tingyi ZhouZhejiang Academy of Medical Sciences
- Hao LiNingbo University
- Jinxuan CaoNingbo University
- Yangying SunNingbo University
- Daodong Pan · correspondingNingbo University; Nanjing Normal University
Abstract
In the present study we purified and identified peptides from broccoli protein hydrolysates and evaluated their angiotensin-converting enzyme (ACE) inhibitory activity in vitro and hypotensive effect in vivo. Three ACE inhibitory peptides were isolated and identified as IPPAYTK, LVLPGELAK, and TFQGPPHGIQVER, and their inhibitory IC50 values were 23.5, 184.0, and 3.4 μM, respectively. We then investigated the effect of gastrointestinal digestion on ACE inhibitory activity. We detected almost two times the ACE inhibitory activity of the peptide LVLPGELAK following simulated digestion than prior to digestion. LVLPGE and LAK, two novel peptides exhibiting high ACE inhibitory activity, were discovered following digestion and possessed IC50 values of 13.5 and 48.0 μM, respectively. The hypotensive effect of the peptides was assessed after oral administration to spontaneous hypertensive rats (SHRs). We found that LVLPGE and LAK demonstrated a significant hypotensive effect in vivo. Protein from broccoli may thus constitute a potential antihypertensive peptide source.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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