Funded in part by U.S. Highbush Blueberry Council
Isolation and Characterization of Blueberry Polyphenolic Components and Their Effects on Gut Barrier Dysfunction
Polewski MA, Esquivel-Alvarado D, Wedde NS, Kruger CG, Reed JD
Journal of agricultural and food chemistry · 30 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Industry funded
- Industry group
- U.S. Highbush Blueberry Council
- Grants
- U.S. Highbush Blueberry Council (MSN194682)
Based on 1 listed funder(s).
Publication
- Published
- 2019-06-14 · J Agric Food Chem · vol. 68 · issue 10 · pp. 2940–2947
- Publisher
- American Chemical Society
- Cited
- 37 citations · more than 90% of similar papers · 2.8× the field average
- References
- 39 works
- Access
- Paywalled
- Research areas
- Biochemical Analysis and Sensing Techniques · Ion Channels and Receptors · Gut microbiota and health
- Keywords
- Proanthocyanidin, Polyphenol, Chemistry, Catechin, Anthocyanin, Food science, Escherichia coli, Fractionation, Flavonoid, Berry, Antimicrobial, Chromatography, Biochemistry, Botany, Biology, Antioxidant, Organic chemistry
- MeSH
- intestinal mucosa, caco-2 cells, humans, escherichia coli, fruit, plant extracts, polyphenols, blueberry plants
5 authors
From US
- Michael A. Polewski · correspondingUniversity of Wisconsin–Madison
- Daniel Esquivel AlvaradoUniversity of Wisconsin–Madison
- Nicholas S. WeddeUniversity of Wisconsin–Madison
- Christian G. KrugerUniversity of Wisconsin–Madison
- Jess Dreher ReedUniversity of Wisconsin–Madison
Abstract
Highbush blueberries contain anthocyanins and proanthocyanidins that have antimicrobial and anti-inflammatory bioactivities. We isolated and characterized three polyphenolic fractions, a total polyphenol fraction (TPF), an anthocyanin-enriched fraction (AEF), and a proanthocyanidin-enriched fraction (PEF), from freeze-dried blueberry powder and evaluated their effects on an in vitro model of gut barrier dysfunction. High-performance liquid chromatography chromatograms illustrate successful fractionation of the blueberry powder into TPF, AEF, and PEF. AEF contained 21 anthocyanins, and PEF contained proanthocyanidin oligomers of (epi)catechin with primarily B-type interflavan bonds. The model uses a strain of Escherichia coli to disrupt a Caco-2 cell monolayer on Transwell inserts. Barrier function was measured by transepithelial electrical resistance (TEER), a marker of membrane permeability. All fractions were able to restore TEER values after an E. coli challenge when compared to the control, while AEF was able to attenuate the E. coli-induced decrease in TEER in a dose-dependent manner.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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