Antimicrobial Resistance and Virulence Genes in Enterococcus faecium and Enterococcus faecalis from Humans and Retail Red Meat
Golob M, Pate M, Kušar D, Dermota U, Avberšek J, Papić B, Zdovc I
BioMed research international · 50 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Cross-sectional study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Javna Agencija za Raziskovalno Dejavnost RS
- Grants
- Javna Agencija za Raziskovalno Dejavnost RS (No. P4-0092); Javna Agencija za Raziskovalno Dejavnost RS (P4-0092)
Based on 1 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2019-05-09 · Biomed Res Int · vol. 2019 · pp. 1–12
- Publisher
- Hindawi Publishing Corporation
- Cited
- 90 citations · more than 97% of similar papers · 7.5× the field average
- Impact
- Top 10% most cited in its field
- References
- 75 works
- Access
- Open access (hybrid journal) · CC-BY
- Research areas
- Probiotics and Fermented Foods · Antimicrobial Resistance in Staphylococcus · Oral microbiology and periodontitis research
- Keywords
- Virulence, Enterococcus faecalis, Enterococcus faecium, Teicoplanin, Biology, Microbiology, Antimicrobial, Broth microdilution, Linezolid, Antibiotic resistance, Enterococcus, Multiple drug resistance, Drug resistance, Gene, Vancomycin, Minimum inhibitory concentration, Antibiotics, Staphylococcus aureus, Bacteria, Genetics
- MeSH
- animals, humans, enterococcus faecalis, enterococcus faecium, anti-bacterial agents, food microbiology, drug resistance, bacterial, slovenia, pork meat
7 authors
From SI
- Majda Golob · correspondingUniversity of Ljubljana
- Mateja PateUniversity of Ljubljana
- Darja KušarUniversity of Ljubljana
- Urška DermotaNational Laboratory of Health, Environment and Food
- Jana AvberšekUniversity of Ljubljana
- Bojan PapićUniversity of Ljubljana
Abstract
The emergence of antimicrobial-resistant and virulent enterococci is a major public health concern. While enterococci are commonly found in food of animal origin, the knowledge on their zoonotic potential is limited. The aim of this study was to determine and compare the antimicrobial susceptibility and virulence traits of Enterococcus faecalis and Enterococcus faecium isolates from human clinical specimens and retail red meat in Slovenia. A total of 242 isolates were investigated: 101 from humans (71 E. faecalis, 30 E. faecium) and 141 from fresh beef and pork (120 E. faecalis, 21 E. faecium). The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of seven common virulence genes was investigated using PCR. In both species, the distribution of several resistance phenotypes and virulence genes was disparate for isolates of different origin. All isolates were susceptible to daptomycin, linezolid, teicoplanin, and vancomycin. In both species, the susceptibility to antimicrobials was strongly associated with a food origin and the multidrug resistance, observed in 29.6% of E. faecalis and 73.3% E. faecium clinical isolates, with a clinical origin (Fisher's exact test). Among meat isolates, in total 66.0% of E. faecalis and E. faecium isolates were susceptible to all antimicrobials tested and 32.6% were resistant to either one or two antimicrobials. In E. faecalis, several virulence genes were significantly associated with a clinical origin; the most common (31.0%) gene pattern included all the tested genes except hyl. In meat isolates, the virulence genes were detected in E. faecalis only and the most common pattern included ace, efaA, and gelE (32.5%), of which gelE showed a statistically significant association with a clinical origin. These results emphasize the importance of E. faecalis in red meat as a reservoir of virulence genes involved in its persistence and human infections with reported severe outcomes.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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