Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression
Melguizo-Rodríguez L, Manzano-Moreno FJ, Illescas-Montes R, Ramos-Torrecillas J, Luna-Bertos E, Ruiz C, García-Martínez O
Nutrients · 32 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Ministerio de Educación, Cultura y Deporte
- University or hospital
- Universidad de Granada
- Government
- Junta de Andalucía
- Grants
- Universidad de Granada (BIO277); Ministerio de Educación, Cultura y Deporte (FPU15-05635); Junta de Andalucía (BIO277)
Based on 3 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2019-07-25 · Nutrients · vol. 11 · issue 8 · p. 1722
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 51 citations · more than 85% of similar papers · 1.8× the field average
- References
- 59 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Cancer, Lipids, and Metabolism · Bone Metabolism and Diseases · Pharmacological Effects of Medicinal Plants
- Keywords
- Olive oil, Osteoblast, Gene expression, Gene, Chemistry, Food science, Biology, Pharmacology, Cell biology, Biochemistry, In vitro
- MeSH
- cell line, osteoblasts, humans, phenols, gene expression regulation, osteogenesis, adolescent, male, bone density conservation agents, olive oil
7 authors
From ES
- Lucía Melguizo‐RodríguezUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Francisco Javier Manzano‐MorenoUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Rebeca Illescas‐MontesUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Javier Ramos‐TorrecillasUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Elvira De Luna‐BertosUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada
- Concepción RuízUniversidad de Granada; Instituto de Investigación Biosanitaria de Granada; Parque Tecnológico de la Salud
Abstract
The phenolic compounds of extra-virgin olive oil can act at various levels to protect individuals against cardiovascular and neurodegenerative diseases, cancer, and osteoporosis, among others. Polyphenols in extra-virgin olive oil can stimulate the proliferation of osteoblasts, modify their antigen profile, and promote alkaline phosphatase synthesis. The objective of this work was to determine the effect of different extra-virgin olive oil phenolic compounds on the gene expression of osteoblast-related markers. The cells of the MG63 osteoblast line were cultured for 24 h with 10-6 M of the phenolic compounds ferulic acid, caffeic acid, coumaric acid, apigenin, or luteolin. The expression of studied markers was quantified using quantitative real-time polymerase chain reaction (q-RT-PCR). The expression by MG63 osteoblasts of growth and differentiation/maturation markers was modified after 24 h of treatment with 10-6 M of the phenolic compounds under study, most of which increased the gene expression of the transforming growth factor β1 (TGF-β1), TGF-β receptor 1,2 and 3 (TGF-βR1, TGF-βR2, TGF-βR3), bone morphogenetic protein 2 and 7 (BMP2, BMP7), run-related transcription factor 2 (RUNX-2), Alkaline phosphatase (ALP), Osteocalcin (OSC), Osterix (OSX), Collagen type I (Col-I) and osteoprotegerin (OPN). The extra-virgin olive oil phenolic compounds may have a beneficial effect on bone by modulating osteoblast physiology, which would support their protective effect against bone pathologies.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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