The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation
Carpi S, Scoditti E, Massaro M, Polini B, Manera C, Digiacomo M, Esposito Salsano J, Poli G, Tuccinardi T, Doccini S, Santorelli FM, Carluccio MA, Macchia M, Wabitsch M, De Caterina R, Nieri P
Nutrients · 85 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- University or hospital
- This work was partially supported by funds from the University of Pisa.
Based on 1 listed funder(s).
Publication
- Published
- 2019-11-21 · Nutrients · vol. 11 · issue 12 · p. 2855
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 108 citations · more than 99% of similar papers · 11.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 127 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Cholesterol and Lipid Metabolism · Atherosclerosis and Cardiovascular Diseases · Natural product bioactivities and synthesis
- Keywords
- Inflammation, Adipocyte, Adipose tissue, Tumor necrosis factor alpha, Angiogenesis, Chemistry, NF-κB, Endocrinology, Internal medicine, Biology, Biochemistry, Medicine
- MeSH
- cells, cultured, adipocytes, humans, inflammation, aldehydes, phenols, nf-kappa b, micrornas, signal transduction, gene expression regulation, exosomes, cyclopentane monoterpenes
16 authors
From IT, DE
- Sara Carpi · correspondingUniversity of Pisa
- Egeria ScodittiIstituto di Fisiologia Clinica; National Research Council
- Marika MassaroIstituto di Fisiologia Clinica; National Research Council
- Beatrice PoliniUniversity of Pisa
- Clementina ManeraUniversity of Pisa
- Maria DigiacomoUniversity of Pisa
Abstract
Inflammation of the adipose tissue plays an important role in the development of several chronic diseases associated with obesity. Polyphenols of extra virgin olive oil (EVOO), such as the secoiridoids oleocanthal (OC) and oleacein (OA), have many nutraceutical proprieties. However, their roles in obesity-associated adipocyte inflammation, the NF-κB pathway and related sub-networks have not been fully elucidated. Here, we investigated impact of OC and OA on the activation of NF-κB and the expression of molecules associated with inflammatory and dysmetabolic responses. To this aim, fully differentiated Simpson-Golabi-Behmel syndrome (SGBS) adipocytes were pre-treated with OC or OA before stimulation with TNF-α. EVOO polyphenols significantly reduced the expression of genes implicated in adipocyte inflammation (IL-1β, COX-2), angiogenesis (VEGF/KDR, MMP-2), oxidative stress (NADPH oxidase), antioxidant enzymes (SOD and GPX), leukocytes chemotaxis and infiltration (MCP-1, CXCL-10, MCS-F), and improved the expression of the anti-inflammatory/metabolic effector PPARγ. Accordingly, miR-155-5p, miR-34a-5p and let-7c-5p, tightly connected with the NF-κB pathway, were deregulated by TNF-α in both cells and exosomes. The miRNA modulation and NF-κB activation by TNF-α was significantly counteracted by EVOO polyphenols. Computational studies suggested a potential direct interaction between OC and NF-κB at the basis of its activity. This study demonstrates that OC and OA counteract adipocyte inflammation attenuating NF-κB activation. Therefore, these compounds could be novel dietary tools for the prevention of inflammatory diseases associated with obesity.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
Community trust
Loading…
How much do you trust this study's findings?
Comments
Sign in to rate, comment on or flag this study.Sign inSomething wrong here?
Flag this study if its information, labels or funding look wrong. An editor reviews every flag.
Sign in to rate, comment on or flag this study.Sign inEducational information about published research. Not medical advice, and not a recommendation to start or stop anything.