Super Critical Fluid Extracted Fatty Acids from Withania somnifera Seeds Repair Psoriasis-Like Skin Lesions and Attenuate Pro-Inflammatory Cytokines (TNF-α and IL-6) Release
Balkrishna A, Nain P, Chauhan A, Sharma N, Gupta A, Ranjan R, Varshney A
Biomolecules · 31 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- People, plus animal or lab work
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2020-01-25 · Biomolecules · vol. 10 · issue 2 · p. 185
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 48 citations · more than 96% of similar papers · 6.2× the field average
- Impact
- Top 10% most cited in its field
- References
- 69 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Phytochemicals and Medicinal Plants · Pharmacological Effects of Medicinal Plants · Complementary and Alternative Medicine Studies
- Keywords
- Withania somnifera, Psoriasis, Inflammation, Tumor necrosis factor alpha, Pharmacology, Cytokine, Proinflammatory cytokine, Chemistry, Anti-inflammatory, Medicine, Immunology, Pathology
- MeSH
- skin, animals, humans, mice, withania, seeds, psoriasis, disease models, animal, inflammation, tetradecanoylphorbol acetate, tumor necrosis factor-alpha, interleukin-6, signal transduction, raw 264.7 cells
7 authors
From IN
- Acharya BalkrishnaPatanjali Research Foundation
- Pradeep NainPatanjali Research Foundation
- Anshul ChauhanPatanjali Research Foundation
- Niti SharmaPatanjali Research Foundation
- Abhishek Kumar GuptaPatanjali Research Foundation
- Ravikant Ranjan · correspondingPatanjali Research Foundation
Abstract
(1) Background: Withania somnifera Dunal (Ashwagandha) is a widely used medicinal herb in traditional medicinal systems with extensive research on various plant parts. Surprisingly, seeds of W. somnifera have never been investigated for their therapeutic potential. (2) Methods: W. somnifera seeds were extracted for fatty acids (WSSO) using super critical fluid extraction, and was analyzed by gas chromatography. Its therapeutic potential in psoriasis-like skin etiologies was investigated using a 12-O tetradecanoyl phorbol 13-acetate (TPA)-induced psoriatic mouse model. Psoriatic inflammation along with psoriatic lesions and histopathological scores were recorded. WSSO was also tested on murine macrophage (RAW264.7), human epidermoid (A431), and monocytic (THP-1) cells, stimulated with TPA or lipo poly-saccharide (LPS) to induce pro-inflammatory cytokine (IL-6 and TNF-α) release. NFκB promoter activity was also measured by luciferase reporter assay. (3) Results: Topical application of WSSO with concurrent oral doses significantly reduced inflammation-induced edema, and repaired psoriatic lesions and associated histopathological scores. Inhibition of pro-inflammatory cytokines release was observed in WSSO-treated A431 and THP-1 cells, along with reduced NFκB expression. WSSO also inhibited reactive nitrogen species (RNS) in LPS-stimulated RAW264.7 cells. (4) Conclusion: Here we show that the fatty acids from W. somnifera seeds have strong anti-inflammatory properties, along with remarkable therapeutic potential on psoriasis-like skin etiologies.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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