Antioxidant Blueberry Anthocyanins Induce Vasodilation via PI3K/Akt Signaling Pathway in High-Glucose-Induced Human Umbilical Vein Endothelial Cells
Huang W, Hutabarat RP, Chai Z, Zheng T, Zhang W, Li D
International journal of molecular sciences · 38 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Natural Science Foundation of China
- University or hospital
- Special Scientific Research Fund of Jiangsu Academy of Agricultural Sciences
- Government
- Natural Science Foundation Program of Jiangsu Province
- Government
- 333 High-level Talent Project Training Funding of Jiangsu Province
- Grants
- National Natural Science Foundation of China (NSFC31601435)
Based on 4 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2020-02-25 · Int J Mol Sci · vol. 21 · issue 5 · p. 1575
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 64 citations · more than 88% of similar papers · 2.1× the field average
- References
- 62 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Heme Oxygenase-1 and Carbon Monoxide · Neutrophil, Myeloperoxidase and Oxidative Mechanisms · Nitric Oxide and Endothelin Effects
- Keywords
- Chemistry, Protein kinase B, NADPH oxidase, Enos, Reactive oxygen species, Biochemistry, Antioxidant, Pharmacology, Malvidin, Nitric oxide synthase, Signal transduction, Biology, Enzyme, Cyanidin
- MeSH
- humans, nitric oxide, anthocyanins, peptidyl-dipeptidase a, xanthine oxidase, protein kinase c, glucose, lipoproteins, ldl, ppar gamma, antioxidants, signal transduction, oxidative stress, vasodilation, proto-oncogene proteins c-akt, phosphatidylinositol 3-kinases, human umbilical vein endothelial cells, blueberry plants, protein kinase c zeta
6 authors
From CN
- Wuyang HuangJiangsu University; Jiangsu Academy of Agricultural Sciences
- Ruth Paulina HutabaratNanjing Normal University; Jiangsu Academy of Agricultural Sciences
- Zhi Hao ChaiJiangsu Academy of Agricultural Sciences
- Tiesong ZhengNanjing Normal University
- Weimin Zhang · correspondingHainan University
- Dajing Li · correspondingJiangsu Academy of Agricultural Sciences
Abstract
Blueberries are rich in antioxidant anthocyanins. The hypotensive effects of blueberry anthocyanins in endothelial cells was investigated here. Pretreatment with blueberry anthocyanin extract, malvidin, malvidin-3-glucoside, and malvidin-3-galactoside significantly ameliorated high-glucose-induced damage by enhancing endogenous antioxidant superoxide dismutase (SOD) and heme oxygenase-1 (HO-1), lowering reactive oxygen species (ROS) generation and NADPH oxidase isoform 4 (NOX4) expression, and increasing the cell vitalities. They also effectively induced a vasodilatory effect by increasing the vasodilator nitric oxide (NO) and its promoters endothelial NO synthase (eNOS) and peroxisome proliferator-activated receptor-γ (PPARγ) levels as well as by decreasing the vasoconstrictor angiotensin-converting enzyme (ACE), xanthine oxidase-1 (XO-1), and low-density lipoprotein (LDL) levels. The activation of phosphoinositide 3-kinase (PI3K)/Akt signaling pathway and the breakdown of protein kinase C zeta (PKCζ) pathway were involved in the bioactivities. The results indicated blueberry anthocyanins protected endothelial function against high-glucose (HG) injury via antioxidant and vasodilatory mechanisms, which could be promising molecules as a hypotensive nutraceutical for diabetes patients.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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