Enhanced bone mineralization using hydroxyapatite-based ceramic bone substitute incorporating Withania somnifera extracts
Qayoom I, Teotia AK, Meena M, Singh P, Mishra A, Singh S, Kumar A
Biomedical materials (Bristol, England) · 17 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Department of Biotechnology, Ministry of Science and Technology, India
- Government
- Department of Science and Technology, Ministry of Science and Technology, India
- Government
- Department of Science and Technology, Ministry of Science and Technology
- Government
- Uchchatar Avishkar Yojana,
- Government
- Department of Biotechnology , Ministry of Science and Technology
- Grants
- Department of Biotechnology, Ministry of Science and Technology, India (BT/IN/SWEDEN/08/AK/2017-18); Department of Science and Technology, Ministry of Science and Technology, India ((#DST/NM/NT-2018/48))
Based on 5 listed funder(s).
Publication
- Published
- 2020-04-09 · Biomed Mater · vol. 15 · issue 5 · p. 055015
- Publisher
- IOP Publishing
- Cited
- 23 citations · more than 72% of similar papers · 1.0× the field average
- References
- 46 works
- Access
- Paywalled
- Research areas
- Bone Tissue Engineering Materials · Orthopaedic implants and arthroplasty · Phytochemicals and Medicinal Plants
- Keywords
- Withania somnifera, Biocompatibility, Chitosan, Materials science, Withaferin A, Calcium, Biomedical engineering, Mineralization (soil science), In vitro, Dentistry, Chemistry, Biochemistry, Medicine, Organic chemistry, Metallurgy
- MeSH
- bone and bones, cell line, tumor, 3t3 cells, osteoblasts, animals, humans, mice, withania, calcium phosphates, hydroxyapatites, durapatite, methanol, chitosan, bone cements, bone substitutes, ceramics, powders, microscopy, electron, scanning, spectroscopy, fourier transform infrared, bone regeneration, cell adhesion, cell differentiation, cell proliferation, calcification, physiologic, particle size, stress, mechanical, in vitro techniques
7 authors
From IN
- Irfan QayoomIndian Institute of Technology Kanpur
- Arun Kumar TeotiaIndian Institute of Technology Kanpur
- Megha MeenaIndian Institute of Technology Kanpur
- Prerna SinghIndian Institute of Technology Kanpur
- Ankita MishraIndian Institute of Technology Kanpur
- Sneha SinghBirla Institute of Technology, Mesra
Abstract
Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits. Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization. In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler. The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts. Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler. In vitro cell adhesion, proliferation and differentiation were evaluated to determine the biocompatibility of the composites. On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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