Walnut (Juglans regia L.) Septum: Assessment of Bioactive Molecules and In Vitro Biological Effects
Rusu ME, Fizesan I, Pop A, Mocan A, Gheldiu AM, Babota M, Vodnar DC, Jurj A, Berindan-Neagoe I, Vlase L, Vlase L, Popa DS
Molecules (Basel, Switzerland) · 38 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
Based on full-text disclosure statement.
Publication
- Published
- 2020-05-07 · Molecules · vol. 25 · issue 9 · p. 2187
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 69 citations · more than 96% of similar papers · 5.0× the field average
- Impact
- Top 10% most cited in its field
- References
- 70 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Nuts composition and effects · Antioxidant Activity and Oxidative Stress · Phytochemicals and Antioxidant Activities
- Keywords
- Juglans, Chemistry, Phytochemical, Cytotoxicity, Antimicrobial, In vitro, Food science, Biochemistry
- MeSH
- cell line, tumor, humans, pseudomonas aeruginosa, salmonella enteritidis, staphylococcus aureus, juglans, nuts, lipase, tocopherols, cholinesterase inhibitors, anti-inflammatory agents, interleukin-8, plant extracts, interleukin-6, antimutagenic agents, antioxidants, anti-infective agents, chromatography, liquid, apoptosis, interleukin-1beta, tandem mass spectrometry, glycoside hydrolase inhibitors
11 authors
From RO
- Marius Emil RusuIuliu Hațieganu University of Medicine and Pharmacy
- Ionel Fizeșan · correspondingIuliu Hațieganu University of Medicine and Pharmacy
- Anca PopIuliu Hațieganu University of Medicine and Pharmacy
- Andrei Mocan · correspondingIuliu Hațieganu University of Medicine and Pharmacy
- Ana-Maria GheldiuIuliu Hațieganu University of Medicine and Pharmacy
- Mihai BabotăIuliu Hațieganu University of Medicine and Pharmacy
Abstract
Walnut (Juglans regia L.) septum represents an interesting bioactive compound source by-product. In our study, a rich phenolic walnut septum extract, previously selected, was further examined. The tocopherol content determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed higher amounts of α-tocopherol compared to γ- and δ-tocopherols. Moreover, several biological activities were investigated. The in vitro inhibiting assessment against acetylcholinesterase, α-glucosidase, or lipase attested a real management potential in diabetes or obesity. The extract demonstrated very strong antimicrobial potential against Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella enteritidis. It also revealed moderate (36.08%) and strong (43.27%) antimutagenic inhibitory effects against TA 98 and TA 100 strains. The cytotoxicity of the extract was assessed on cancerous (A549, T47D-KBluc, MCF-7) and normal (human gingival fibroblasts (HGF)) cell lines. Flow cytometry measurements confirmed the cytotoxicity of the extract in the cancerous cell lines. Additionally, the extract demonstrated antioxidant activity on all four cell types, as well as anti-inflammatory activity by lowering the inflammatory cytokines (interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-1 β (IL-1β)) evaluated in HGF cells. To the best of our knowledge, most of the cellular model analyses were performed for the first time in this matrix. The results prove that walnut septum may be a potential phytochemical source for pharmaceutical and food industry.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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