Stored RBC metabolism as a function of caffeine levels
D'Alessandro A, Fu X, Reisz JA, Kanias T, Page GP, Stone M, Kleinman S, Zimring JC, Busch M, Recipient Epidemiology and Donor Evaluation Study-III (REDS III)
Transfusion · 28 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Controlled clinical trial (indexed by PubMed)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- National Institutes of Health
- Government
- National Heart, Lung, and Blood Institute
- Government
- National Institute of General Medical Sciences
- Government
- NHLBI NIH HHS
- Government
- NIGMS NIH HHS
- Government
- NIH HHS
- Grants
- National Heart, Lung, and Blood Institute (R01HL146442); National Institute of General Medical Sciences (RM1 GM131968); National Institutes of Health (1s10od021641-01a1); National Heart, Lung, and Blood Institute (R01HL148151); National Heart, Lung, and Blood Institute (RM1GM131968); National Heart, Lung, and Blood Institute (R21 HL150032); National Heart, Lung, and Blood Institute (R01 HL149714); National Heart, Lung, and Blood Institute (HHSN2682011); National Heart, Lung, and Blood Institute (S10OD021641)
Based on 6 listed funder(s).
Publication
- Published
- 2020-05-11 · Transfusion · vol. 60 · issue 6 · pp. 1197–1211
- Publisher
- Wiley
- Cited
- 31 citations · more than 92% of similar papers · 3.2× the field average
- Impact
- Top 10% most cited in its field
- References
- 76 works
- Access
- Open access (repository copy)
- Research areas
- Coffee research and impacts · Forensic Toxicology and Drug Analysis · Erythrocyte Function and Pathophysiology
- Keywords
- Caffeine, Function (biology), Metabolism, Chemistry, Biochemistry, Medicine, Biology, Internal medicine, Cell biology
- MeSH
- erythrocytes, humans, caffeine, xanthine, blood preservation, glycolysis, coffee, adult, female, male, pentose phosphate pathway, metabolomics
10 authors
From US, CA
- Angelo D’Alessandro · correspondingVitalant; Vitalant Research Institute; University of Colorado Denver
- Xiaoyun FuBloodworks Northwest
- Julie A. ReiszUniversity of Colorado Denver
- Tamir KaniasVitalant; Vitalant Research Institute; University of Colorado Denver
- Grier P. PageRTI International
- Mars StonePacific Research Institute; Vitalant; Vitalant Research Institute
Abstract
Background
Coffee consumption is extremely common in the United States. Coffee is rich with caffeine, a psychoactive, purinergic antagonist of adenosine receptors, which regulate red blood cell energy and redox metabolism. Since red blood cell (purine) metabolism is a critical component to the red cell storage lesion, here we set out to investigate whether caffeine levels correlated with alterations of energy and redox metabolism in stored red blood cells.
Study design and methods
We measured the levels of caffeine and its main metabolites in 599 samples from the REDS-III RBC-Omics (Recipient Epidemiology Donor Evaluation Study III Red Blood Cell-Omics) study via ultra-high-pressure-liquid chromatography coupled to high-resolution mass spectrometry and correlated them to global metabolomic and lipidomic analyses of RBCs stored for 10, 23, and 42 days.
Results
Caffeine levels positively correlated with increased levels of the main red cell antioxidant, glutathione, and its metabolic intermediates in glutathione-dependent detoxification pathways of oxidized lipids and sugar aldehydes. Caffeine levels were positively correlated with transamination products and substrates, tryptophan, and indole metabolites. Expectedly, since caffeine and its metabolites belong to the family of xanthine purines, all xanthine metabolites were significantly increased in the subjects with the highest levels of caffeine. However, high-energy phosphate compounds ATP and DPG were not affected by caffeine levels, despite decreases in glucose oxidation products-both via glycolysis and the pentose phosphate pathway.
Conclusion
Though preliminary, this study is suggestive of a beneficial correlation between the caffeine levels and improved antioxidant capacity of stored red cells.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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