Blueberry-Derived Exosome-Like Nanoparticles Counter the Response to TNF-α-Induced Change on Gene Expression in EA.hy926 Cells
De Robertis M, Sarra A, D'Oria V, Mura F, Bordi F, Postorino P, Fratantonio D
Biomolecules · 146 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2020-05-10 · Biomolecules · vol. 10 · issue 5 · p. 742
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 187 citations · more than 97% of similar papers · 5.5× the field average
- Impact
- Top 10% most cited in its field
- References
- 52 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Extracellular vesicles in disease · MicroRNA in disease regulation · Circular RNAs in diseases
- Keywords
- microRNA, Cell biology, Microvesicles, Biology, KEGG, Gene, Gene expression, Exosome, Transcriptome, Biochemistry
- MeSH
- cell line, humans, inflammation, reactive oxygen species, tumor necrosis factor-alpha, micrornas, rna, messenger, cell death, cell survival, endocytosis, gene expression regulation, base sequence, oxidative stress, cytoprotection, nanoparticles, exosomes, gene ontology, blueberry plants
7 authors
From IT
- Mariangela De RobertisInstitute of Biomembranes, Bioenergetics and Molecular Biotechnologies; National Research Council; University of Bari Aldo Moro
- Angelo SarraRoma Tre University; Sapienza University of Rome
- Valentina DoriaBambino Gesù Children's Hospital; Istituti di Ricovero e Cura a Carattere Scientifico
- Francesco MuraSapienza University of Rome
- F. BordiSapienza University of Rome
- P. PostorinoSapienza University of Rome
Abstract
Exosome-like nanoparticles (ELNs) are attracting interest as important vehicles of intercellular communication, both in prokaryotes and eukaryotes. Recently, dietary nanoparticles similar to mammalian exosomes have attracted attention for these features. In particular they appear to be relevant in the modulation of several cellular processes as well as candidate carriers of bioactive molecules (proteins, lipids, and nucleic acids, including miRNAs) with therapeutic value. Herein, we investigated the cellular uptake of blueberry-derived ELNs (B-ELNs) by a human stabilized endothelial cell line (EA.hy926) and the ability of B-ELNs to modulate the expression of inflammatory genes as the response of tumor necrosis factor-α (TNF-α). Our results indicate that 1) EA.hy926 cells internalize B-ELNs in a dose-dependent manner; 2) pretreatment with B-ELNs counters TNF-α-induced reactive oxygen species (ROS) generation and loss of cell viability and modulates the differential expression of 29 genes (fold change > 1.5) induced by TNF-α compared to control; 3) pathway analysis reveals their involvement in a total of 340 canonical pathways, 121 KEGG pathways, and 121 GO Biological processes; and 4) the intersection between differentially expressed (DE) genes and miRNAs contained in B-ELNs unveils a set of candidate target genes, such as prostaglandin I2 synthase (PTGIS), mitogen-activated protein kinase 14 (MAPK14), and phosphodiesterase 7A (PDE7A), for ELNs-contained cargo. In conclusion, our study indicates that B-ELNs can be considered candidate therapeutic carriers of bioactive compounds potentially able to protect vascular system against various stressors.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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