The effect of dietary nitrate supplementation on the speed-duration relationship in mice with sickle cell disease
Ferguson SK, Redinius KM, Harral JW, Pak DI, Swindle DC, Hirai DM, Blackwell JR, Jones AM, Stenmark KR, Buehler PW, Irwin DC
Journal of applied physiology (Bethesda, Md. : 1985) · 16 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Government
- National Institutes of Health
- Government
- National Heart, Lung, and Blood Institute
- Government
- National Institute of General Medical Sciences
- Government
- National Institute of Diabetes and Digestive and Kidney Diseases
- Government
- NIGMS NIH HHS
- University or hospital
- Colorado Nutrition and Obesity Research Center
- Government
- NIH HHS
- Government
- HHS | National Institutes of Health
- Government
- NHLBI NIH HHS
- Government
- NIDDK NIH HHS
- Grants
- National Heart, Lung, and Blood Institute (P01-HL-014985); National Institute of Diabetes and Digestive and Kidney Diseases (P30 DK048520); National Heart, Lung, and Blood Institute (T32HL007171); National Institute of General Medical Sciences (P20GM 103466); National Heart, Lung, and Blood Institute (R01 HL125642); National Institutes of Health (1r01hl125642-01a1); National Institutes of Health (DK048520); National Institutes of Health (P30DK048520); National Institutes of Health (T32-Hl007171)
Based on 10 listed funder(s).
Publication
- Published
- 2020-07-23 · J Appl Physiol (1985) · vol. 129 · issue 3 · pp. 474–482
- Publisher
- American Physiological Society
- Cited
- 17 citations · more than 75% of similar papers · 1.0× the field average
- References
- 65 works
- Access
- Free to read
- Research areas
- Hemoglobinopathies and Related Disorders · Iron Metabolism and Disorders · Peroxisome Proliferator-Activated Receptors
- Keywords
- Disease, Medicine, Dietary Nitrate, Sickle cell anemia, Internal medicine, Cell, Endocrinology, Nitrate, Treadmill, Anemia, Physiology, Biology, Nitrite, Biochemistry
- MeSH
- animals, humans, mice, beta vulgaris, anemia, sickle cell, nitrates, double-blind method, oxygen consumption, exercise tolerance, dietary supplements
11 authors
From US, GB
- Scott K. Ferguson · correspondingUniversity of Hawaii at Hilo; University of Colorado Anschutz; University of Colorado Denver
- Katherine M. RediniusUniversity of Colorado Anschutz; University of Colorado Denver
- Julie Wright HarralUniversity of Colorado Anschutz; University of Colorado Denver
- David I. PakUniversity of Colorado Anschutz; University of Colorado Denver
- Delaney C. SwindleUniversity of Colorado Anschutz; University of Colorado Denver
- Daniel M. HiraiPurdue University West Lafayette
Abstract
Sickle cell disease (SCD) causes exercise intolerance likely due to impaired skeletal muscle function and low nitric oxide (NO) bioavailability. Dietary nitrate improves hemodynamic and metabolic control during exercise in humans and animals. The purpose of this investigation was to assess the impact of nitrate supplementation on exercise capacity as measured by the running speed to exercise duration relationship [critical speed (CS)]in mice with SCD. We tested the hypothesis that nitrate supplementation via beetroot juice (BR) would attenuate the exercise intolerance observed in mice with SCD. Ten wild-type (WT) and 18 Berkley sickle-cell mice (BERK) received water (WT: n = 10, BERK: n = 10) or nitrate-rich BR (BERK+BR: n = 8, nitrate dose 1 mmol/kg/day) for 5 days. Following the supplementation period, all mice performed 3-5 constant-speed treadmill tests that resulted in exhaustion within 1.5 to 20 min. Time to exhaustion vs. treadmill speed was fit to a hyperbolic model to determine CS. CS was significantly lower in BERK vs. WT and BERK+BR with no significant difference between WT and BERK+BR (WT: 36.6 ± 1.6, BERK: 23.8 ± 1.5, BERK+BR: 31.1 ± 2.1 m/min, P NEW & NOTEWORTHY Sickle cell disease compromises muscle O2 delivery resulting in exercise intolerance. Dietary nitrate supplementation increases skeletal muscle blood flow during exercise and may improve exercise capacity in a mouse model of sickle cell disease. We investigated the effects of dietary nitrate supplementation on exercise tolerance in a mouse model of sickle cell disease using the treadmill speed-duration relationship (critical speed). Mice with sickle cell disease provided with a dietary nitrate supplement had a critical speed not significantly different from healthy wild-type mice.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
Community trust
Loading…
How much do you trust this study's findings?
Comments
Sign in to rate, comment on or flag this study.Sign inSomething wrong here?
Flag this study if its information, labels or funding look wrong. An editor reviews every flag.
Sign in to rate, comment on or flag this study.Sign inEducational information about published research. Not medical advice, and not a recommendation to start or stop anything.