Time course of human skeletal muscle nitrate and nitrite concentration changes following dietary nitrate ingestion
Kadach S, Piknova B, Black MI, Park JW, Wylie LJ, Stoyanov Z, Thomas SM, McMahon NF, Vanhatalo A, Schechter AN, Jones AM
Nitric oxide : biology and chemistry · 35 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (classified by our AI screen)
- Studied in
- People
- Main outcome
- Mechanisms only
- Intake measured by
- Not stated
Who paid for it
- Funding
- Independent funding
- University or hospital
- University of Exeter
- University or hospital
- University of Queensland
- Government
- National Institutes of Health
- Government
- Biotechnology and Biological Sciences Research Council
- Government
- Intramural NIH HHS
- Grants
- Biotechnology and Biological Sciences Research Council (BB/S020632/1); National Institutes of Health (Z99 DK999999); Biotechnology and Biological Sciences Research Council (BB/S020632/1)
Based on 5 listed funder(s).
Publication
- Published
- 2022-01-13 · Nitric Oxide · vol. 121 · pp. 1–10
- Publisher
- Elsevier BV
- Cited
- 44 citations · more than 96% of similar papers · 4.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 49 works
- Access
- Open access (hybrid journal) · CC-BY-NC-ND
- Research areas
- Nitric Oxide and Endothelin Effects · Cardiovascular and exercise physiology · Pharmacological Effects and Assays
- Keywords
- Ingestion, Skeletal muscle, Internal medicine, Nitrite, Endocrinology, Nitric oxide, Chemistry, Bolus (digestion), Urine, Nitrate, Vastus lateralis muscle, Animal science, Medicine, Biology
- MeSH
- muscle, skeletal, humans, nitrates, nitrites, time factors, dietary supplements, adult, female, male, young adult
11 authors
From GB, US, AU
- Stefan KadachUniversity of Exeter
- Barbora PiknovaNational Institutes of Health; National Institute of Diabetes and Digestive and Kidney Diseases
- Matthew I. BlackUniversity of Exeter
- Ji Won ParkNational Institutes of Health; National Institute of Diabetes and Digestive and Kidney Diseases
- Lee J. WylieUniversity of Exeter
- Zdravko StoyanovUniversity of Exeter
Abstract
Dietary nitrate (NO3-) ingestion can be beneficial for health and exercise performance. Recently, based on animal and limited human studies, a skeletal muscle NO3- reservoir has been suggested to be important in whole body nitric oxide (NO) homeostasis. The purpose of this study was to determine the time course of changes in human skeletal muscle NO3- concentration ([NO3-]) following the ingestion of dietary NO3-. Sixteen participants were allocated to either an experimental group (NIT: n = 11) which consumed a bolus of ∼1300 mg (12.8 mmol) potassium nitrate (KNO3), or a placebo group (PLA: n = 5) which consumed a bolus of potassium chloride (KCl). Biological samples (muscle (vastus lateralis), blood, saliva and urine) were collected shortly before NIT or PLA ingestion and at intervals over the course of the subsequent 24 h. At baseline, no differences were observed for muscle [NO3-] and [NO2-] between NIT and PLA (P > 0.05). In PLA, there were no changes in muscle [NO3-] or [NO2-] over time. In NIT, muscle [NO3-] was significantly elevated above baseline (54 ± 29 nmol/g) at 0.5 h, reached a peak at 3 h (181 ± 128 nmol/g), and was not different to baseline from 9 h onwards (P > 0.05). Muscle [NO2-] did not change significantly over time. Following ingestion of a bolus of dietary NO3-, skeletal muscle [NO3-] increases rapidly, reaches a peak at ∼3 h and subsequently declines towards baseline values. Following dietary NO3- ingestion, human m. vastus lateralis [NO3-] expressed a slightly delayed pharmacokinetic profile compared to plasma [NO3-].
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY-NC-ND).
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