Determination of tissue contributions to the circulating lipid pool in cold exposure via systematic assessment of lipid profiles
Jain R, Wade G, Ong I, Chaurasia B, Simcox J
Journal of lipid research · 33 citations
How it was studied
- Design
- Animal study (classified by our AI screen)
- Studied in
- Animals
- Main outcome
- Health markers and function
Who paid for it
- Funding
- Independent funding
- Nonprofit
- American Diabetes Association
- Government
- U.S. Department of Agriculture
- University or hospital
- Wisconsin Alumni Research Foundation
- University or hospital
- University of Wisconsin-Madison
- University or hospital
- Washington University in St. Louis
- Government
- National Institutes of Health
- Unclassified
- Office of the Director
- University or hospital
- College of Engineering, University of Wisconsin-Madison
- University or hospital
- Office of the Vice Chancellor for Research and Graduate Education, University of Wisconsin-Madison
- Government
- NIH Office of the Director
- Government
- National Cancer Institute
- Government
- National Institute of Diabetes and Digestive and Kidney Diseases
- University or hospital
- Division of Graduate Education
- Government
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- Government
- NIDDK NIH HHS
- Government
- NICHD NIH HHS
- Government
- NCI
- Government
- NIDDK
- Government
- USDA
- Grants
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK020579); Eunice Kennedy Shriver National Institute of Child Health and Human Development (K12 HD101368); U.S. Department of Agriculture (2019-67018-29250); National Institute of Diabetes and Digestive and Kidney Diseases (R01DK115824); American Diabetes Association (7-21-JDF-033); National Institute of Diabetes and Digestive and Kidney Diseases (DK115824); National Institute of Diabetes and Digestive and Kidney Diseases (DK124326); National Cancer Institute (K12HD101368); National Institute of Diabetes and Digestive and Kidney Diseases (R01 DK124326); National Institute of Diabetes and Digestive and Kidney Diseases (RC2DK125961); National Institutes of Health (DK124326); National Institutes of Health (P30-DK020579); National Institutes of Health (DK115824); National Institutes of Health (K12HD101368); National Institutes of Health (RC2DK125961)
Based on 19 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2022-03-15 · J Lipid Res · vol. 63 · issue 7 · p. 100197
- Publisher
- Elsevier BV
- Cited
- 37 citations · more than 96% of similar papers · 4.0× the field average
- Impact
- Top 10% most cited in its field
- References
- 55 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Adipose Tissue and Metabolism · Lipid metabolism and biosynthesis · Metabolomics and Mass Spectrometry Studies
- Keywords
- Lipidome, Lipidomics, Adipose tissue, Lipid metabolism, Biology, Internal medicine, Endocrinology, Chemistry, Bioinformatics, Medicine
- MeSH
- humans, diabetes mellitus, type 2, lipids, fasting, thermogenesis, adipose tissue, brown, cold temperature, lipidomics
5 authors
From US
- Raghav JainUniversity of Wisconsin–Madison
- Gina WadeUniversity of Wisconsin–Madison
- Irene M. OngUniversity of Wisconsin–Madison
- Bhagirath ChaurasiaUniversity of Iowa; Fraternal Order of Eagles
- Judith Simcox · correspondingUniversity of Wisconsin System; University of Wisconsin–Madison
Abstract
Plasma lipid levels are altered in chronic conditions such as type 2 diabetes and cardiovascular disease as well as during acute stresses such as fasting and cold exposure. Advances in MS-based lipidomics have uncovered a complex plasma lipidome of more than 500 lipids that serve functional roles, including as energy substrates and signaling molecules. This plasma lipid pool is maintained through regulation of tissue production, secretion, and uptake. A major challenge in understanding the lipidome complexity is establishing the tissues of origin and uptake for various plasma lipids, which is valuable for determining lipid functions. Using cold exposure as an acute stress, we performed global lipidomics on plasma and in nine tissues that may contribute to the circulating lipid pool. We found that numerous species of plasma acylcarnitines (ACars) and ceramides (Cers) were significantly altered upon cold exposure. Through computational assessment, we identified the liver and brown adipose tissue as major contributors and consumers of circulating ACars, in agreement with our previous work. We further identified the kidney and intestine as novel contributors to the circulating ACar pool and validated these findings with gene expression analysis. Regression analysis also identified that the brown adipose tissue and kidney are interactors with the plasma Cer pool. Taken together, these studies provide an adaptable computational tool to assess tissue contribution to the plasma lipid pool. Our findings have further implications in understanding the function of plasma ACars and Cers, which are elevated in metabolic diseases.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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