Funded in part by Egg Nutrition Center, American Egg Board/Egg Nutrition Center
Comparison between Egg Intake versus Choline Supplementation on Gut Microbiota and Plasma Carotenoids in Subjects with Metabolic Syndrome
Thomas MS, DiBella M, Blesso CN, Malysheva O, Caudill M, Sholola M, Cooperstone JL, Fernandez ML
Nutrients · 17 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- Randomized controlled trial (indexed by PubMed)
- Studied in
- People
- Main outcome
- Health markers and function
- Intake measured by
- Food provided by researchers
Who paid for it
- Funding
- Industry funded
- Industry group
- Egg Nutrition Center
- Industry group
- American Egg Board/Egg Nutrition Center
- Authors
- At least one author declares a financial tie to industry
Based on 2 listed funder(s) and full-text disclosure statement.
Publication
- Published
- 2022-03-11 · Nutrients · vol. 14 · issue 6 · p. 1179
- Publisher
- Multidisciplinary Digital Publishing Institute
- Cited
- 23 citations · more than 93% of similar papers · 3.7× the field average
- Impact
- Top 10% most cited in its field
- References
- 89 works
- Access
- Open access (journal) · CC-BY
- Research areas
- Nutritional Studies and Diet · Gut microbiota and health · Folate and B Vitamins Research
- Keywords
- Lutein, Zeaxanthin, Carotenoid, Choline, Xanthophyll, Gut flora, Chemistry, Lachnospiraceae, Food science, Biology, Internal medicine, Animal science, Endocrinology, Biochemistry, Medicine, 16S ribosomal RNA
- MeSH
- humans, choline, carotenoids, rna, ribosomal, 16s, dietary supplements, eggs, gastrointestinal microbiome, metabolic syndrome
8 authors
From US
- Minu Sara ThomasUniversity of Connecticut
- Marissa DiBellaUniversity of Connecticut
- Christopher N. BlessoUniversity of Connecticut
- Olga V. MalyshevaCornell University
- Marie A. CaudillCornell University
- Maria J. ShololaThe Ohio State University
Abstract
We previously demonstrated that intake of three eggs/d for 4 weeks increased plasma choline and decreased inflammation in subjects with metabolic syndrome (MetS). The purpose of the current study was to further explore the effects of phosphatidylcholine (PC) provided by eggs versus a choline bitartrate (CB) supplement on the gut microbiota, trimethylamine N-oxide (TMAO) formation, and plasma carotenoids lutein and zeaxanthin in MetS. This randomized, controlled crossover clinical trial included 23 subjects with MetS. Following a washout period of 2 weeks without consuming any choline-containing foods, subjects were randomly allocated to consume either three eggs/d or a CB supplement for 4 weeks (both diets had a choline equivalent of 400 mg/day). DNA was extracted from stool samples to sequence the 16S rRNA gene region for community analysis. Operational taxonomic units (OTUs) and the α-diversity of the community were determined using QIIME software. Plasma TMAO, methionine, betaine, and dimethylglycine (DMG) were quantified by stable isotope dilution liquid chromatography with tandem mass spectrometry. Plasma carotenoids, lutein, and zeaxanthin were measured using reversed-phase high-performance liquid chromatography. There were significant increases in plasma lutein and zeaxanthin after egg intake compared to the baseline or intake of CB supplement (p 0.05). Additionally, while diet intervention had no effects on microbiota diversity measures or relative taxa abundances, a correlation between bacterial biodiversity and HDL was observed. Following egg intake, the observed increases in plasma lutein and zeaxanthin may suggest additional protection against oxidative stress, a common condition in MetS.
Abstract via Europe PMC. Copyright remains with the authors or publisher (CC BY).
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