Unveiling the effect of Withania somnifera on neuronal cytoarchitecture and synaptogenesis: A combined in vitro and network pharmacology approach
Mitra S, Munni YA, Dash R, Sultana A, Moon IS
Phytotherapy research : PTR · 11 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Independent funding
- Government
- Korean Ministry of Science and ICT
Based on 1 listed funder(s).
Publication
- Published
- 2022-04-20 · Phytother Res · vol. 36 · issue 6 · pp. 2524–2541
- Publisher
- Wiley
- Cited
- 14 citations · more than 91% of similar papers · 3.4× the field average
- Impact
- Top 10% most cited in its field
- References
- 65 works
- Access
- Paywalled
- Research areas
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection · Neuroscience and Neural Engineering
- Keywords
- Synaptogenesis, Neurotrophin, Withania somnifera, Pharmacology, Nerve growth factor, Tropomyosin receptor kinase A, Neuroscience, Hippocampal formation, Biology, Medicine, Receptor, Biochemistry
- MeSH
- neurons, humans, withania, memory disorders, nerve growth factor, plant extracts, molecular docking simulation, network pharmacology
5 authors
From KR, BD
- Sarmistha MitraDongguk University
- Yeasmin Akter MunniDongguk University
- Raju DashDongguk University
- Armin SultanaBGC Trust University Bangladesh
- Il Soo Moon · correspondingDongguk University
Abstract
Withania somnifera (WS), is known for its remarkable contribution in herbal medicine and Ayurveda, which is therapeutically applied to improve memory and anxiety in patients. However, the pharmacological details of this plant on memory boosting yet remained undefined. This study provides mechanistic insights on the effect of ethanol solution extract of the whole plant of WS (WSEE) on neuritogenesis by combining in vitro and in silico network pharmacology approaches. WSEE promoted significant neuronal growth through early differentiation, axodendritic arborization, and synaptogenesis on primary hippocampal neurons. The network pharmacological study confirmed that the neuritogenic activity is potentially mediated by modulating the neurotrophin signaling pathway, where NRTK1 (TrkA) was revealed as the primary target of WS secondary metabolites. This neurotrophic activity of WSEE was significantly stifled by the presence of TrkA inhibitor, which further confirms the TrkA-dependent activity of WSEE. In addition, a molecular docking study suggested steroidal lactones present in the WS might act as nerve growth factor (NGF)-mimetics, activating TrkA by binding to the NGF-binding domain. As a whole, the findings of the study suggest a significant role of WSEE on neuritogenesis and its potential to function as a therapeutic agent and in drug designing for the prevention and treatment of memory-related neurological disorders.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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