Cholecalciferol induces apoptosis via autocrine metabolism in epidermoid cervical cancer cells
Bhoora S, Pillay TS, Punchoo R
Biochemistry and cell biology = Biochimie et biologie cellulaire · 8 citations
Review labels
Neutral facts our review recorded about how this study was done. They describe method, never whether we like the result.
How it was studied
- Design
- In vitro/mechanistic study (classified by our AI screen)
- Studied in
- Cells or lab samples
- Main outcome
- Mechanisms only
Who paid for it
- Funding
- Funding not disclosed
Publication
- Published
- 2022-06-20 · Biochem Cell Biol · vol. 100 · issue 5 · pp. 387–402
- Publisher
- NRC Research Press
- Cited
- 9 citations · more than 78% of similar papers · 1.2× the field average
- References
- 70 works
- Access
- Paywalled
- Research areas
- Vitamin D Research Studies · Neuroendocrine Tumor Research Advances · Neuroblastoma Research and Treatments
- Keywords
- Cholecalciferol, CYP24A1, Calcitriol receptor, Calcitriol, Apoptosis, Vitamin D and neurology, Autocrine signalling, Biology, Endocrinology, Internal medicine, Viability assay, Bcl-xL, Calcifediol, Molecular biology, Programmed cell death, Biochemistry, Receptor, Medicine
- MeSH
- humans, gentian violet, trypan blue, cholecalciferol, calcifediol, calcitriol, 25-hydroxyvitamin d3 1-alpha-hydroxylase, caspases, phosphatidylserines, vitamin d, receptors, calcitriol, apoptosis, uterine cervical neoplasms, female, vitamin d3 24-hydroxylase, biomarkers
3 authors
From ZA
- Sachin BhooraUniversity of Pretoria
- T.S. PillayNational Health Laboratory Service; University of Cape Town; University of Pretoria
- Rivak PunchooNational Health Laboratory Service; University of Pretoria
Abstract
The anti-cancer effects of vitamin D are of fundamental interest. Cholecalciferol is sequentially hydroxylated endogenously to calcidiol and calcitriol. Here, SiHa epidermoid cervical cancer cells were treated with cholecalciferol (10-2600 nmol/L). Cell count and viability were assayed using Crystal Violet and Trypan Blue, respectively. Apoptosis was assessed using flow cytometry for early and late biomarkers along with brightfield microscopy and transmission electron microscopy. Autocrine vitamin D metabolism was analysed by reverse transcription-quantitative PCR and immunoblotting for activating enzymes: 25-hydroxylases (CYP2R1 and CYP27A1) and 1α-hydroxylase (CYP27B1), the catabolic 24-hydroxylase (CYP24A1), and the vitamin D receptor (VDR). Data were analysed using one-way ANOVA and Bonferroni post-hoc test, and p p = 0.011) and viability (p p = 0.0145), and phosphatidylserine externalisation (p = 0.0439), terminal caspase activity (p = 0.0025), and nuclear damage (p = 0.004) increased. Microscopy showed classical features of apoptosis. Gene and protein expression were concordant. Immunoblots revealed increased CYP2R1 (p = 0.021), VDR (p = 0.04), and CYP24A1 (p = 0.0274) and decreased CYP27B1 (p = 0.031). The authors conclude that autocrine activation of cholecalciferol to calcidiol may mediate VDR signalling of growth inhibition and apoptosis in SiHa cells.
Abstract via Europe PMC. Copyright remains with the authors or publisher.
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